Gene Expression Data
Assay Details
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Assay
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Reference:
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J:91012
Kurokawa D, et al., Regulation of Otx2 expression and its functions in mouse epiblast and anterior neuroectoderm. Development. 2004 Jul;131(14):3307-17
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3056068
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Gene symbol:
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Six3
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Gene name:
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sine oculis-related homeobox 3
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Probe:
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Six3 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6Ba:
embryonic day 7.5
(more  )
Specimen
6Ba:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: future brain |
Present |
Regionally restricted |
6B a
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Expression is in anterior neurectoderm.
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Specimen
6Bb:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Otx2tm2.1Imat/Otx2tm7Sia
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: future brain |
Present |
Regionally restricted |
6B b
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Expression in anterior neurectoderm was induced in these mutants.
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Specimen
6Bc:
embryonic day 8.5
(more )
Specimen
6Bc:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Present |
Regionally restricted |
6B c
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Expression is in anterior neurectoderm.
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Specimen
6Bd:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Otx2tm2.1Imat/Otx2tm7Sia
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future brain |
Present |
Regionally restricted |
6B d
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Expression in anterior neurectoderm is reduced.
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Analysis Tools