Gene Expression Data
Assay Details
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Assay
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Reference:
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J:89188
Raft S, et al., Suppression of neural fate and control of inner ear morphogenesis by Tbx1. Development. 2004 Apr;131(8):1801-12
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3044710
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Gene symbol:
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Lfng
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Gene name:
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LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
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Probe:
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pMFR1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3A:
(close  )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * FVB * SJL
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Mutant Allele(s):
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Tbx1tm1Bem/Tbx1tm1Bem
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: otocyst epithelium |
Present |
Regionally restricted |
3A
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There is ectopic expression throughout the posterolateral otocyst.
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Specimen
3B:
embryonic day 10.0
(more  )
Specimen
3B:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * FVB * SJL
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: otocyst epithelium |
Present |
Regionally restricted |
3B
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Expression is absent from the Tbx1-positive posterolateral regions.
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Specimen
3E:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * FVB * SJL
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Mutant Allele(s):
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Tbx1tm1Bem/Tbx1tm1Bem
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst epithelium |
Present |
Regionally restricted |
3E
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There is ectopic expression throughout the posterolateral otocyst.
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Specimen
3F:
embryonic day 10.5
(more )
Specimen
3F:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * FVB * SJL
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst epithelium |
Present |
Regionally restricted |
3F
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Expression is absent from the Tbx1-positive posterolateral regions.
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Specimen
6A:
embryonic day 9.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * FVB * SJL
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst epithelium |
Present |
Regionally restricted |
6A
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There is a medial-lateral intensity gradient and borders of dorsal and medial expression.
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * FVB * SJL
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Mutant Allele(s):
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Tbx1tm1Bem/Tbx1tm1Bem
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst epithelium |
Present |
Regionally restricted |
6B
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The medial-lateral intensity gradient and the dorsal and medial borders of expression are preserved in this mutant.
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Specimen
6C:
embryonic day 9.5
(more )
Specimen
6C:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * FVB * SJL
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst epithelium |
Present |
Regionally restricted |
6C
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Expression is absent from the Tbx1-positive posterolateral regions.
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Specimen
6D:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * FVB * SJL
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Mutant Allele(s):
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Tbx1tm1Bem/Tbx1tm1Bem
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: otocyst epithelium |
Present |
Regionally restricted |
6D
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There is ectopic expression throughout the posterolateral otocyst.
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