Probe:	Ran cDNA
Probe preparation:	labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS10: ectoplacental cone	Present		1A

Structure	Level	Pattern	Image	Note
TS10: ectoplacental cone	Present		1B

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present		1C
TS11: neural fold	Present		1C
TS11: future spinal cord neural plate	Present	Regionally restricted	1C	Expression is in the neural groove.
TS11: blood island	Present		1C
TS11: ectoplacental cone	Present		1C

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present		1D
TS11: neural fold	Present		1D
TS11: future spinal cord neural plate	Present	Regionally restricted	1D	Expression is in the neural groove.
TS11: blood island	Present		1D
TS11: ectoplacental cone	Present		1D

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Strong		1E
TS11: extraembryonic component	Weak	Regionally restricted	1E

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present		1G
TS12: future prosencephalon	Strong	Regionally restricted	1G	Strongest expression is in regions around neuropores.
TS12: future spinal cord	Strong	Regionally restricted	1G	Strongest expression is in regions around neuropores.
TS12: trunk somite	Weak		1G

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		2A
TS13: future prosencephalon	Strong	Regionally restricted	2A	Strongest expression is in regions around neuropores.
TS13: future spinal cord	Strong	Regionally restricted	2A	Strongest expression is in regions around neuropores.
TS13: neural crest	Present		2A
TS13: 1st branchial arch	Present	Regionally restricted	2A	Expression is in the mandibular and maxillary components.
TS13: 2nd branchial arch	Present		2A

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		2A1
TS13: future prosencephalon	Strong	Regionally restricted	2A1	Stongest expression is in regions around neuropores.
TS13: optic eminence	Present		2A1

Structure	Level	Pattern	Image	Note
TS13: future spinal cord	Present		2A2
TS13: trunk somite	Weak		2A2

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		2A3
TS13: future prosencephalon	Strong	Regionally restricted	2A3	Stongest expression is in regions around neuropores.

Structure	Level	Pattern	Image	Note
TS16: eye	Present		2B
TS16: otocyst	Present		2B
TS16: limb	Strong	Graded	2B inset , 2B	A proximal to distal gradation. The strongest expression is at the distal tip of the limb buds. Expression is absent under the apical ectodermal ridge.
TS16: 1st branchial arch mandibular component	Present		2B
TS16: 1st branchial arch maxillary component	Present		2B
TS16: 2nd branchial arch	Present		2B
TS16: latero-nasal process	Present		2B

Structure	Level	Pattern	Image	Note
TS14: caudal neuropore	Present		2C
TS14: rostral neuropore	Present		2C
TS14: optic vesicle	Present		2C
TS14: otic pit	Present	Regionally restricted	2C	Expression is in the otic vesicle.
TS14: otic placode	Present	Regionally restricted	2C	Expression is in the otic vesicle.
TS14: 1st branchial arch mandibular component	Present		2C
TS14: 1st branchial arch maxillary component	Present		2C
TS14: 2nd branchial arch	Present		2C

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		2D

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present		2D1
TS15: otocyst	Present		2D1
TS15: 1st branchial arch mandibular component	Present		2D1
TS15: 1st branchial arch maxillary component	Present		2D1
TS15: 2nd branchial arch	Present		2D1

Structure	Level	Pattern	Image	Note
TS19: brain ventricular layer	Present	Regionally restricted	3A	Expression is in the germ layer within the encephalic vesicle walls.
TS19: future spinal cord	Present		3A
TS19: eye	Present	Regionally restricted	3A	Expression is in the eye including the otpic vesicle.

Structure	Level	Pattern	Image	Note
TS19: hindbrain lateral wall	Present	Regionally restricted	3B	Expression is in the germ layer within the encephalic vesicle walls.
TS19: telencephalon ventricular layer	Present	Regionally restricted	3B	Expression is in the germ layer within the encephalic vesicle walls.
TS19: future spinal cord	Present		3B
TS19: eye	Present	Regionally restricted	3B	Expression is in the eye including the otpic vesicle.

Structure	Level	Pattern	Image	Note
TS19: lung	Present		3C

Structure	Level	Pattern	Image	Note
TS19: liver	Present		3D

Structure	Level	Pattern	Image	Note
TS19: limb	Strong	Graded	3E	A proximal to distal gradation. The strongest expression is at the distal tip of the limb buds.

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present		3F
TS19: trunk sclerotome	Strong		3F	Expression is in the sclerotomic material forming the centrum of the axis.

Structure	Level	Pattern	Image	Note
TS19: footplate mesenchyme	Present	Regionally restricted	3G	Expression is in the dorsal and ventral mesenchyme.
TS19: future leg mesenchyme	Present	Regionally restricted	3G	Expression is in the dorsal and ventral mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Present	Regionally restricted	3H	Expression is in the germ layer within the encephalic vesicle walls.
TS19: future spinal cord	Present		3H
TS19: dorsal root ganglion	Present		3H

Structure	Level	Pattern	Image	Note
TS19: nephric duct	Present	Regionally restricted	3I	Expression is in the mesonephric duct.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Regionally restricted	4A	Expression is present in the roof.
TS21: midbrain	Present	Regionally restricted	4A	Expression is present in the roof.
TS21: spinal cord	Present		4A

Structure	Level	Pattern	Image	Note
TS21: liver	Present		4B
TS21: metanephros	Present		4B

Structure	Level	Pattern	Image	Note
TS21: lung	Present		4C