Reporter:	lacZ
	Direct Detection
Assay notes:	Authors state that specimens were fixed with 0.4% paraformaldehyde.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Present		1a	Staining in Sertoli cells restricted to residual bodies.
TS28: germ cell of testis		Strong	Single cells	1a	Most intense staining appeared to be associated with maturing germ cells; particularly intense in primary spermatocytes.
TS28: primary spermatocyte		Strong	Single cells	1a	Most intense staining appeared to be associated with maturing germ cells; particularly intense in primary spermatocytes.
TS28: interstitium of the testis		Absent		1a

Structure	Level	Pattern	Image	Note
TS28: testis	Weak			Authors report faint punctate staining. As the first cycle of spermatogenesis progressed, intensity of staining increased.

Structure	Level	Pattern	Image	Note
TS28: testis	Weak			Authors report identical staining pattern as seen in heterozygotes.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present	Single cells	1c	Authors state that staining is confined to developing eggs.

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present	Single cells	1e	Authors report staining throughout egg cytoplasm as well as in an intense blue spot close to the germinal vesicle.

Structure	Level	Pattern	Image	Note
TS28: metanephros	Present	Regionally restricted	2a	Staining restricted to some proximal convoluted tubules; not observed within all tubules. Authors report patchy staining probably arepresents a fixation artifact. No staining in renal corpuscles.

Structure	Level	Pattern	Image	Note
TS28: heart atrium	Present		2c	Authors report patchy staining probably arepresents a fixation artifact.
TS28: heart ventricle	Present		2c	Authors report patchy staining probably arepresents a fixation artifact.

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	2e	Widespread staining; strongest in cell bodies, including olfactory bulb, striatum, hippocamplus, fasciculus retroflexus, lateral hypothalamus, cortex and cerebellum.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		3_7.5 dpc	Authors report expression associated with the embryo.
TS11: extraembryonic component	Absent		3_7.5 dpc

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		3_8.5 dpc	Authors report widespread embryonic expression.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		3_9.5 dpc	Authors report widespread embryonic expression.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		3_10.5 dpc	Authors report widespread embryonic expression.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	3_11.5 dpc	Authors state that expression became more restricted.
TS17: limb	Present	Regionally restricted	3_11.5 dpc	Intense staining on apical surface of developing limbs.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	3_12.5 dpc	Authors state that expression became more restricted.
TS20: limb	Present	Regionally restricted	3_12.5 dpc	Intense staining on apical surface of developing limbs.

Structure	Level	Pattern	Image	Note
TS21: embryo	Present	Regionally restricted	3_13.5 dpc	Authors state that expression became more restricted.
TS21: limb	Present	Regionally restricted	3_13.5 dpc	Intense staining on apical surface of developing limbs.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Regionally restricted	3_14.5 dpc	Authors state that expression became more restricted.
TS22: integumental system	Present	Regionally restricted	3_14.5 dpc	Staining concentrated in the developing hair follicle.
TS22: outer ear	Present		3_14.5 dpc	Authors report that staining is concentrated in the outer ear.