Gene Expression Data
Assay Details
Assay
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Reference:
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J:79392
Bai CB, et al., Gli2, but not Gli1, is required for initial Shh signaling and ectopic activation of the Shh pathway. Development. 2002 Oct;129(20):4753-61
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2676443
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe45
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3A:
embryonic day 7.5
(more )
Specimen
3A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo mesoderm |
Present |
Regionally restricted |
3A
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Authors report expression starts at E7.5 in the midline mesoderm of the head process.
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TS11: notochordal process |
Present |
Regionally restricted |
3A
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Authors report expression starts at E7.5 in the midline mesoderm of the head process.
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Specimen
3B:
embryonic day 8.0
(more )
Specimen
3B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: embryo |
Present |
Regionally restricted |
3B
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Expression expands posteriorly along the midline into the node.
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TS12: future midbrain |
Present |
Regionally restricted |
3B
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By the headfold stage, expression is initiated in the anterior midbrain of the neural ectoderm.
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Specimen
3C:
embryonic day 8.5
(more )
Specimen
3C:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 8.5
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Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: future hindbrain |
Present |
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3C
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Expression expands anteriorly into the ventral forebrain and posteriorly along the spinal cord.
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TS13: future midbrain |
Present |
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3C
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Expression expands anteriorly into the ventral forebrain and posteriorly along the spinal cord.
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TS13: future prosencephalon floor plate |
Present |
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3C
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Expression expands anteriorly into the ventral forebrain and posteriorly along the spinal cord.
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TS13: future spinal cord |
Present |
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3C
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Expression expands anteriorly into the ventral forebrain and posteriorly along the spinal cord.
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Specimen
3D:
embryonic day 9.5
(more )
Specimen
3D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
|
Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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