Probe:	Hoxc8 probe8
Probe preparation:	DNA

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent
TS11: extraembryonic component	Absent

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent
TS11: extraembryonic component	Absent

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted	2A wt	Expression detected in the in the posterior region of the embryo.
TS12: allantois	Present		2A wt

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent		2A EEneo

Structure	Level	Pattern	Image	Note
TS13: head paraxial mesenchyme	Present	Regionally restricted	2B wt	Strongest expression is in posterior region; expression domains are extended to more anterior regions.
TS13: neural tube	Present	Regionally restricted	2B wt	Strongest expression is in posterior region; expression domains are extended to more anterior regions.
TS13: trunk paraxial mesenchyme	Present	Regionally restricted	2B wt	Strongest expression is in posterior region; expression domains are extended to more anterior regions.

Structure	Level	Pattern	Image	Note
TS13: head paraxial mesenchyme	Present	Regionally restricted	2B EEneo	Authors report a delay of expression in both neural tube and paraxial mesoderm; expression domains are restricted to the caudal region and are relatively lower in intensity compared with wild-type embryos.
TS13: neural tube	Present	Regionally restricted	2B EEneo	Authors report a delay of expression in both neural tube and paraxial mesoderm; expression domains are restricted to the caudal region and are relatively lower in intensity compared with wild-type embryos.
TS13: trunk paraxial mesenchyme	Present	Regionally restricted	2B EEneo	Authors report a delay of expression in both neural tube and paraxial mesoderm; expression domains are restricted to the caudal region and are relatively lower in intensity compared with wild-type embryos.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	2C wt	Anterior boundaries of peak expression in the neural tube and paraxial mesenchyme are at the level of somites 10 and 14, respectively.
TS14: trunk paraxial mesenchyme	Present	Regionally restricted	2C wt	Anterior boundaries of peak expression in the neural tube and paraxial mesenchyme are at the level of somites 10 and 14, respectively.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Weak	Regionally restricted	2C EEneo	Anterior limit of expression in the neural tube is established correctly, but the expression level is very low.
TS14: tail bud	Weak	Regionally restricted	2C EEneo	Expression in paraxial mesenchyme is weak and restricted to the unsegmented tail bud region.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	2D wt	Expression in neural tube between somites 9 and 15.
TS16: trunk paraxial mesenchyme	Present	Regionally restricted	2D wt	Expression domain within paraxial mesoderm is from somites 15 to 23.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present		2D EEneo	Expression level within the neural tube is normal.
TS16: trunk paraxial mesenchyme	Present	Regionally restricted	2D EEneo	Anterior boundary of expression at somite 15; posterior boundary is anteriorized to somite 21.
TS16: somite 16	Strong		2D EEneo	Strongest expression between somites 16 and 19.
TS16: somite 17	Strong		2D EEneo	Strongest expression between somites 16 and 19.
TS16: somite 18	Strong		2D EEneo	Strongest expression between somites 16 and 19.
TS16: somite 19	Strong		2D EEneo	Strongest expression between somites 16 and 19.

Structure	Level	Pattern	Image	Note
TS18: trunk paraxial mesenchyme	Present	Regionally restricted	2E wt

Structure	Level	Pattern	Image	Note
TS18: trunk paraxial mesenchyme	Present	Regionally restricted	2E EEneo	Failure to form a normal posterior boundary in the paraxial mesoderm continues.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	2F wt
TS19: trunk paraxial mesenchyme	Present	Regionally restricted	2F wt
TS19: trunk somite	Weak	Regionally restricted	2F wt	Expression intensity in somite 22 and 23 becomes significantly weaker.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	2F EEneo	Expression domains within both neural tube and somite are comparable to wild-type embryos.
TS19: trunk paraxial mesenchyme	Present	Regionally restricted	2F EEneo	Expression domains within both neural tube and somite are comparable to wild-type embryos.
TS19: trunk somite	Strong	Regionally restricted	2F EEneo	Expression level at somites 22 and 23 are stronger than in wild-type.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted	3A wt	Expression detected in the in the posterior region of the embryo.
TS12: allantois	Present		3A wt

Structure	Level	Pattern	Image	Note
TS12: allantois	Present		3A EElox	Expression is detected in the allantois and posterior region of the embryo.
TS12: embryo	Absent		3A EElox	Expression has not spread to more anterior regions.

Structure	Level	Pattern	Image	Note
TS13: head paraxial mesenchyme	Present	Regionally restricted	3B wt
TS13: neural tube	Present	Regionally restricted	3B wt
TS13: trunk paraxial mesenchyme	Present	Regionally restricted	3B wt

Structure	Level	Pattern	Image	Note
TS13: head paraxial mesenchyme	Present	Regionally restricted	3B EElox	Authors state that expression patterns in both neural tube and paraxial mesoderm are identical to wild-type.
TS13: neural tube	Present	Regionally restricted	3B EElox	Authors state that expression patterns in both neural tube and paraxial mesoderm are identical to wild-type.
TS13: trunk paraxial mesenchyme	Present	Regionally restricted	3B EElox	Authors state that expression patterns in both neural tube and paraxial mesoderm are identical to wild-type.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	3C wt	Anterior boundaries of peak expression in the neural tube and somites are at the level of somites 10 and 14, respectively.
TS14: trunk paraxial mesenchyme	Present	Regionally restricted	3C wt	Anterior boundaries of peak expression in the neural tube and somites are at the level of somites 10 and 14, respectively.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	3C EElox	Authors state that expression patterns in both neural tube and paraxial mesoderm are identical to wild-type.
TS14: trunk paraxial mesenchyme	Present	Regionally restricted	3C EElox	Authors state that expression patterns in both neural tube and paraxial mesoderm are identical to wild-type.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	3D wt	Expression in neural tube between somites 9 and 15.
TS15: trunk paraxial mesenchyme	Present	Regionally restricted	3D wt	Expression domain within paraxial mesoderm is from somites 15 to 23.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	3D EElox	Anterior boundary of expression is identical to wild-type.
TS16: trunk paraxial mesenchyme	Present	Regionally restricted	3D EElox	Anterior boundary of expression is identical to wild-type; posterior boundary is anteriorized to somite 21
TS16: embryo	Weak	Regionally restricted	3D EElox	Overall level of expression appears lower compared with wild-type embryos.

Structure	Level	Pattern	Image	Note
TS18: trunk paraxial mesenchyme	Present	Regionally restricted	3E wt

Structure	Level	Pattern	Image	Note
TS18: trunk paraxial mesenchyme	Present	Regionally restricted	3E EElox	Authors report the same somite expression pattern is observed.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	3F wt
TS19: trunk paraxial mesenchyme	Present	Regionally restricted	3F wt
TS19: trunk somite	Weak	Regionally restricted	3F wt	Expression intensity in somite 22 and 23 becomes significantly weaker.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Weak		3F EElox	Expression domains within both neural tube and somites are comparable to that of wild-type embryo. Expression intensity is within somites is lower compared with wild-type embryos.
TS19: trunk paraxial mesenchyme	Weak		3F EElox	Expression domains within both neural tube and somites are comparable to that of wild-type embryo. Expression intensity is within somites is lower compared with wild-type embryos.