Fgf9 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:76230 Hajihosseini MK, et al., Expression patterns of fibroblast growth factors-18 and -20 in mouse embryos is suggestive of novel roles in calvarial and limb development. Mech Dev. 2002 Apr;113(1):79-83
Assay type:	RNA in situ
MGI Accession ID:	MGI:2665487
Gene symbol:	Fgf9
Gene name:	fibroblast growth factor 9

Probe:	Fgf9 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

2J ©

Specimen 2J: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: vault of skull	Present	Regionally restricted	2J	Expression is at the rims of the calvarial bones.
TS25: suture	Present		2J

2K ©

Specimen 2K: embryonic day 17.5 (more

)

Structure	Level	Image
TS25: inter-parietal bone	Present	2K
TS25: parietal bone	Present	2K
TS25: lambdoid suture	Present	2K

2L ©

Specimen 2L: embryonic day 17.5 (more

)
Note: Double labeled: purple - Fgf9; orange - Spp1 (osteopontin; assay MGI:2665358). Tissue was embedded in 2% agar and a 100-micron-thick vibratome section was cut.

Structure	Level	Image
TS25: inter-parietal bone	Present	2L
TS25: parietal bone	Present	2L
TS25: lambdoid suture	Present	2L

3M ©

Specimen 3M: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: limb	Present		3M

3N ©

Specimen 3N: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: limb	Present		3N

3O ©

Specimen 3O: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: limb	Present		3O

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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