Probe:	Eomes probe3
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS5: polar trophectoderm	Weak		1A right/top	Expression is throughout the lateral and polar trophectoderm.
TS5: mural trophectoderm	Weak	Single cells	1A right/top	Expression is in the trophoblastic giant cells.
TS5: inner cell mass	Absent		1A right/top

Structure	Level	Pattern	Image	Note
TS5: polar trophectoderm	Weak		1A right/bottom	Expression is throughout the lateral and polar trophectoderm.
TS5: mural trophectoderm	Weak	Single cells	1A right/bottom	Expression is in the trophoblastic giant cells.
TS5: inner cell mass	Absent		1A right/bottom

Structure	Level	Pattern	Image	Note
TS9: extraembryonic ectoderm	Present		1B left embryo
TS9: primitive streak	Present		1B left embryo
TS9: embryo mesoderm	Present	Regionally restricted	1B left embryo	Expression is in the lateral wings of the newly formed mesoderm.

Structure	Level	Pattern	Image	Note
TS9: extraembryonic ectoderm	Present		1B middle embryo
TS9: primitive streak	Present		1B middle embryo
TS9: embryo mesoderm	Present	Regionally restricted	1B middle embryo	Expression is in the lateral wings of the newly formed mesoderm.

Structure	Level	Pattern	Image	Note
TS9: extraembryonic ectoderm	Present		1B right embryo
TS9: primitive streak	Present		1B right embryo
TS9: embryo mesoderm	Present	Regionally restricted	1B right embryo	Expression is in the lateral wings of the newly formed mesoderm.

Structure	Level	Pattern	Image	Note
TS9: primitive streak	Present		1B section (bottom)
TS9: embryo mesoderm	Present	Regionally restricted	1B section (bottom)	Expression is in the lateral wings of the newly formed mesoderm.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		1C left embryo
TS11: embryo mesoderm	Present	Regionally restricted	1C left embryo	Expression is in the lateral wings of the newly formed mesoderm.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		1C middle embryo
TS11: embryo mesoderm	Present	Regionally restricted	1C middle embryo	Expression is in the lateral wings of the newly formed mesoderm.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent		1C right embryo	In this slightly advanced embryo the expression in the lateral wings of the newly formed mesoderm is absent.
TS12: primitive streak	Absent		1C right embryo

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent

Structure	Level	Pattern	Image	Note
TS17: telencephalon mantle layer	Present		1D right

Structure	Level	Pattern	Image	Note
TS19: telencephalon mantle layer	Present		1E Meomes

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex mantle layer	Present		1F
TS20: cerebral cortex	Present	Regionally restricted	1F	There is an additional stripe of expression in the anterior/ventral part of the cortex.
TS20: forelimb digit 4 mesenchyme	Present	Regionally restricted	1F	Expression is in a spot at the base of the fourth digit.
TS20: hindlimb digit 4 mesenchyme	Present	Regionally restricted	1F	Expression is in a spot at the base of the fourth digit.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex mantle layer	Present		1G top section

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex mantle layer	Present		1G bottom section

Structure	Level	Pattern	Image	Note
TS20: forelimb digit 4 mesenchyme	Present	Regionally restricted	1H right	Expression is in a spot at the base of the fourth digit.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit 4 mesenchyme	Present	Regionally restricted	1H section (top right)	Expression is in a spot at the base of the fourth digit.