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Gene Expression Data
Assay Details
Assay
Reference: J:54209 Ciruna BG, et al., Expression of the T-box gene Eomesodermin during early mouse development. Mech Dev. 1999 Mar;81(1-2):199-203
Assay type: RNA in situ
MGI Accession ID: MGI:2450703
Gene symbol: Eomes
Gene name: eomesodermin
Probe: Eomes cDNA4
Probe preparation: Antisense RNA
Assay notes: Identical results were obtained with the Eomes cDNA5 probe (MGI:2450621).
Results
Specimen 1A: embryonic day 5.0 (more )
Note: Egg cylinder stage embryo.
Structure Level Pattern Image Note
TS7: polar trophectoderm Strong 1A Expression was strong in the extraembryonic ectoderm.
Specimen 1B: embryonic day 6.5 (more )
Note: Pre-streak stage embryo.
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 1B Expression was detected in the epiblast at the extraembryonic/embryonic junction.
TS9: extraembryonic ectoderm Present 1B
Specimen 1C: embryonic day 6.75 (more )
Note: Early streak stage embryo.
Structure Level Pattern Image Note
TS9: primitive streak Present 1C
TS9: embryo mesoderm Present 1C Expression in nascent mesoderm.
TS9: embryo Present Regionally restricted 1C Expression was detected in the posterior third of the epiblast.
TS9: extraembryonic ectoderm Present 1C
Specimen 1D: embryonic day 7.0 (more )
Note: Mid-streak stage embryo.
Structure Level Pattern Image Note
TS10: extraembryonic ectoderm Present 1D
TS10: primitive streak Present 1D
TS10: anterior visceral endoderm Present Regionally restricted 1D Expression was detected in the distal and anterior visceral endoderm.
TS10: embryo Present Regionally restricted 1D Expression was detected in the posterior third of the epiblast.
Specimen 1E: embryonic day 7.0 (more )
Note: Mid-streak stage embryo. The fix used prior to sectioning the whole mounts was 3.7% formaldehyde overnight.
Structure Level Pattern Image Note
TS10: extraembryonic ectoderm Present 1E
Specimen 1F: embryonic day 7.0 (more )
Note: Mid-streak stage embryo. The fix used prior to sectioning the whole mounts was 3.7% formaldehyde overnight.
Structure Level Pattern Image Note
TS10: primitive streak Present 1F
TS10: embryo mesoderm Present 1F
Specimen 1G: embryonic day 7.0 (more )
Note: Mid-streak stage embryo. The fix used prior to sectioning the whole mounts was 3.7% formaldehyde overnight.
Structure Level Pattern Image Note
TS10: extraembryonic ectoderm Present 1G
TS10: primitive streak Present 1G
TS10: anterior visceral endoderm Present Regionally restricted 1G Expression was detected in the distal and anterior visceral endoderm.
TS10: embryo Present Regionally restricted 1G Expression was detected in the posterior third of the epiblast.
Specimen 2A: embryonic day 7.0 (more )
Note: Mid-late streak stage embryo.
Structure Level Pattern Image Note
TS10: primitive streak Present Regionally restricted 2A Expression was restricted to the anterior primitive streak.
TS10: extraembryonic ectoderm Present 2A
Specimen 2C: embryonic day 7.5 (more )
Note: Late streak stage embryo.
Structure Level Pattern Image Note
TS10: primitive streak Present Regionally restricted 2C Expression was restricted to the anterior primitive streak.
TS10: extraembryonic ectoderm Present 2C
Specimen 2Cinsert: embryonic day 7.5 (more )
Note: Late streak stage embryo. The fix used prior to sectioning the whole mounts was 3.7% formaldehyde overnight.
Structure Level Pattern Image Note
TS10: primitive streak Present 2C inset
TS10: embryo mesoderm Present Regionally restricted 2C inset Expression was detected in the mesodermal cells most proximal to the primitive streak.
Specimen 2D: embryonic day 8.0 (more )
Note: Early head-fold stage embryo.
Structure Level Pattern Image Note
TS11: chorion Present 2D
TS11: embryo Absent 2D
Specimen 3A: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS18: telencephalon Strong Regionally restricted 3A Expression was absent from the dorsal midline.
Specimen 3B: embryonic day 10.5 (more )
Note: The fix used prior to sectioning the whole mounts was 3.7% formaldehyde overnight.
Structure Level Pattern Image Note
TS18: telencephalon mantle layer Present Regionally restricted 3B , 3C Expression was detected in the superficial layer of the telencephalic vesicle but was absent from the dorsal midline.
Specimen 3D: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS18: telencephalon Present Regionally restricted 3D Expression was absent from the dorsal midline.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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