Probe:	msr/apj cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS12: anterior cardinal vein	Strong		2A
TS12: head somite	Strong		2A
TS12: trunk somite	Strong		2A
TS12: vitelline vein	Strong		2A
TS12: allantois	Weak		2A
TS12: ectoplacental cone	Weak		2A
TS12: future brain	Absent		2A
TS12: heart	Absent		2A
TS12: yolk sac	Absent		2A

Structure	Level	Pattern	Image	Note
TS14: head somite	Present	Regionally restricted	2B	Expression in anterior somites is restricted to the medio-ventral region.
TS14: trunk somite	Present	Regionally restricted	2B	Expression is homogeneous in posterior somites and restricted to the medio-ventral region of anterior somites.
TS14: common cardinal vein	Present		2B
TS14: arterial system	Present		2B	Expression in the forming large vessels, brain vessels, and extending into the branchial arches, and intersomitic vessels.
TS14: venous system	Present		2B	Expression in the forming large vessels, brain vessels, and extending into the branchial arches, and intersomitic vessels.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Strong		2C
TS15: forelimb bud	Strong		2C
TS15: future forebrain	Strong		2C
TS15: arterial system	Present		2C	Expression in the forming large vessels, brain vessels, and extending into the branchial arches, and intersomitic vessels.
TS15: venous system	Present		2C	Expression in the forming large vessels, brain vessels, and extending into the branchial arches, and intersomitic vessels.
TS15: heart	Weak		2C
TS15: gut	Weak		2C	Expression in visceral organs.

Structure	Level	Pattern	Image	Note
TS15: cardinal vein	Present	Regionally restricted	2D	Expression in the endothelial lining of the left cardinal vein.

Structure	Level	Pattern	Image	Note
TS15: bulbus cordis caudal half endocardial lining	Present	Regionally restricted	2E
TS15: bulbus cordis rostral half endocardial lining	Present	Regionally restricted	2E
TS15: common atrial chamber endocardial lining	Present	Regionally restricted	2E
TS15: primitive ventricle endocardial lining	Present	Regionally restricted	2E
TS15: anterior cardinal vein	Present	Regionally restricted	2E	Expression in the endothelial lining.
TS15: dorsal aorta	Present	Regionally restricted	2E	Expression in the endothelial lining.
TS15: trunk somite	Present		2E

Structure	Level	Pattern	Image	Note
TS15: trunk sclerotome	Present		2F
TS15: trunk dermomyotome	Absent		2F
TS15: forelimb bud	Strong	Regionally restricted	2F	Expression in vascular structures and mesenchyme.
TS15: posterior cardinal vein	Strong	Regionally restricted	2F	Expression in the endothelial lining.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present	Regionally restricted	2G	Expression in the core mesenchyme.

Structure	Level	Pattern	Image	Note
TS12: anterior cardinal vein	Present		3A left	Expression in the forming vessels, more notably in intersomitic vessels, and posterior cardinal vein.
TS12: vitelline vein	Present		3A left	Expression in the forming vessels, more notably in intersomitic vessels, and posterior cardinal vein.
TS12: arterial system	Present		3A left	Expression in the forming vessels, more notably in intersomitic vessels, and posterior cardinal vein.
TS12: venous system	Present		3A left	Expression in the forming vessels, more notably in intersomitic vessels, and posterior cardinal vein.

Structure	Level	Pattern	Image	Note
TS14: 1st branchial arch	Strong		3B left
TS14: forelimb bud	Present		3B left
TS14: prosencephalon	Present		3B left
TS14: tail bud	Present		3B left
TS14: arterial system	Present	Regionally restricted	3B left	Expression in intersomitic vessels, absent in the capillaries of the head.
TS14: venous system	Present	Regionally restricted	3B left	Expression in intersomitic vessels, absent in the capillaries of the head.
TS14: heart	Absent		3B left

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present		3C left
TS15: posterior cardinal vein	Present		3C left
TS15: trunk somite	Present		3C left
TS15: extraembryonic component	Present	Regionally restricted	3C left	Absent in the yolk sac.