Probe:	Desrt cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS10: extraembryonic component	Present		1A
TS10: embryo	Absent		1A
TS10: ectoplacental cone	Present	Regionally restricted	1A	Expression was detected in the trophoblast giant cells.

Structure	Level	Pattern	Image	Note
TS12: foregut diverticulum	Present		1B
TS12: intermediate mesenchyme	Present		1B
TS12: future hindbrain neural crest	Present	Regionally restricted	1B	Expression was detected in the premigratory neural crest.
TS12: future midbrain neural crest	Present	Regionally restricted	1B	Expression was detected in the premigratory neural crest.

Structure	Level	Pattern	Image	Note
TS13: branchial arch	Present		1C
TS13: foregut diverticulum	Present		1C
TS13: hindgut diverticulum	Present		1C
TS13: oral region	Present	Regionally restricted	1C	Expression was detected in the oro-nasal-pharyngeal tissue.
TS13: extraembryonic component	Present	Regionally restricted	1C	Expression was detected in the allantois.
TS13: nephrogenic cord	Present	Regionally restricted	1C	Expression was present in the nephrogenic cord.
TS13: future hindbrain	Present	Regionally restricted	1C	Expression was present in the premigratory neural crest.
TS13: future midbrain neural crest	Present	Regionally restricted	1C	Expression was present in the premigratory neural crest.
TS13: head somite	Present	Regionally restricted	1C	Expression was detected in a distinct caudal band corresponding to the anterior region of the segmenting somite.
TS13: trunk somite	Present	Regionally restricted	1C	Expression was detected in a distinct caudal band corresponding to the anterior region of the segmenting somite.

Structure	Level	Pattern	Image	Note
TS14: nephrogenic cord	Present	Regionally restricted	1D	Expression was detected in the nephrogenic mesoderm.
TS14: forelimb bud ectoderm	Present	Regionally restricted	1D
TS14: forelimb bud mesenchyme	Present	Regionally restricted	1D	Expression was detected in the proximal mesenchyme.
TS14: foregut	Present	Regionally restricted	1D	Expression was detected in the foregut portal endoderm.
TS14: future midbrain neural crest	Present	Regionally restricted	1D	Expression was detected in the premigratory neural crest.
TS14: future hindbrain	Present	Regionally restricted	1D	Expression was detected in the hindbrain premigratory neural crest.
TS14: head somite	Present	Regionally restricted	1D	Expression was detected in a distinct caudal band corresponding to the anterior region of the segmenting somite.
TS14: trunk somite	Present	Regionally restricted	1D	Expression was detected in a distinct caudal band corresponding to the anterior region of the segmenting somite.

Structure	Level	Pattern	Image	Note
TS16: liver	Present		1E
TS16: mesonephric tubule	Present		1E
TS16: otocyst epithelium	Present		1E
TS16: limb	Present	Regionally restricted	1E	Expression was detected in the rostral and caudal mesenchyme of the limb bud.
TS16: 1st branchial arch	Present	Regionally restricted	1E	Expression was detected in the ectoderm and mesenchyme.
TS16: 2nd branchial arch	Present	Regionally restricted	1E	Expression was detected in the ectoderm and mesenchyme.
TS16: arterial system	Present	Regionally restricted	1E	Expression was detected in the truncus arteriosus.
TS16: heart	Absent		1E
TS16: esophagus	Strong		1E
TS16: lung	Strong		1E
TS16: thyroid primordium	Strong		1E
TS16: trachea	Strong		1E
TS16: oral region cavity	Strong	Regionally restricted	1E	Expression was strong in the lining.
TS16: foregut gland	Strong	Regionally restricted	1E	Expression was detected in the thymus.
TS16: nasal pit	Present	Regionally restricted	1E	Expression was detected in the olfactory placode.
TS16: trunk dermomyotome	Present		1E

Structure	Level	Pattern	Image	Note
TS15: mesonephric tubule	Present		1F
TS15: limb	Present	Regionally restricted	1F	Expression was detected in the rostral and caudal mesenchyme of the limb bud.
TS15: hindgut	Present	Regionally restricted	1F	Expression was detected in the endoderm.

Structure	Level	Pattern	Image	Note
TS14: forelimb bud ectoderm	Present	Regionally restricted	2A	Expression was detected in the surface ectoderm.
TS14: forelimb bud mesenchyme	Present	Regionally restricted	2A	Expression was detected in the proximal mesenchyme.
TS14: arterial system	Present	Regionally restricted	2A	Expression was detected in the truncus arteriosus.
TS14: heart	Absent		2A

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Regionally restricted	2B	Expression was detected in the rostral and caudal mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	2C	Expression was detected in the rostral and caudal mesenchyme of the prospective arm region but absent from the handplate.
TS17: forelimb bud ectoderm	Absent		2C

Structure	Level	Pattern	Image	Note
TS19: handplate mesenchyme	Present	Regionally restricted	2D	Expression was detected broadly in the distal mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: foot mesenchyme	Present	Regionally restricted	2E	Expression was detected broadly in the distal mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: hand mesenchyme	Present	Regionally restricted	2F	Expression was detected broadly in the distal mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: hindlimb interdigital region	Present		2G

Structure	Level	Pattern	Image	Note
TS17: endolymphatic appendage	Present		3A
TS17: endolymphatic appendage epithelium	Present		3A
TS17: liver	Present		3A
TS17: otocyst epithelium	Present		3A
TS17: trunk dermomyotome	Present		3A
TS17: 1st branchial arch	Present	Regionally restricted	3A	Expression was detected in the ectoderm and mesenchyme.
TS17: 2nd branchial arch	Present	Regionally restricted	3A	Expression was detected in the ectoderm and mesenchyme.
TS17: esophagus	Strong		3A
TS17: lung	Strong		3A
TS17: thyroid primordium	Strong		3A
TS17: trachea	Strong		3A
TS17: oral region cavity	Strong	Regionally restricted	3A	Expression was detected in the lining.
TS17: foregut gland	Strong	Regionally restricted	3A	Expression was detected in the thymus.

Structure	Level	Pattern	Image	Note
TS19: endolymphatic appendage	Present		3B
TS19: trunk dermomyotome	Present		3B
TS19: head mesenchyme	Present	Regionally restricted	3B	Expression was present in the mesenchyme underlying the ectoderm covering the forebrain and face.
TS19: embryo ectoderm	Present	Regionally restricted	3B	Expression was detected in the ectoderm covering the forebrain and face.

Structure	Level	Pattern	Image	Note
TS20: endolymphatic appendage	Present		3C
TS20: external auditory meatus	Present		3C
TS20: tail dermomyotome	Present		3C
TS20: embryo mesenchyme	Present	Regionally restricted	3C	Expression was detected in the mesenchyme underlying the ectoderm covering the forebrain and face.
TS20: head	Present	Regionally restricted	3C	Expression was detected in the ectoderm covering the forebrain and face.
TS20: nasal cavity	Present	Regionally restricted	3C	Expression was detected in the olfactory placodes and nasal pits.

Structure	Level	Pattern	Image	Note
TS21: lower eyelid	Present		3D
TS21: upper eyelid	Present		3D
TS21: nasal cavity	Present	Regionally restricted	3D	Expression was detected in the nasal pits.
TS21: trunk	Present	Regionally restricted	3D	Expression was detected in the hair follicles.
TS21: vibrissa	Present	Regionally restricted	3D	Expression was detected in the hair follicles.
TS21: reproductive system	Present	Regionally restricted	3D	Expression was detected in the surface ectoderm and mesenchyme of the genital tubercle.

Structure	Level	Pattern	Image	Note
TS19: liver	Present

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted		Expression was detected in the hypothalamus.