Gene Expression Data
Assay Details
Assay
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Reference:
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J:80018
Przemeck GK, et al., Node and midline defects are associated with left-right development in Delta1 mutant embryos. Development. 2003 Jan;130(1):3-13
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2449023
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Gene symbol:
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Lfng
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Gene name:
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LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
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Probe:
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Lfng cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
Not Shown:
embryonic day 7.0
(more )
Note: Mid gastrulation.
Specimen
Not Shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 7.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Mid gastrulation.
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Specimen
4I top view:
embryonic day 7.5
(more )
Specimen
4I top view:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo |
Present |
Regionally restricted |
4I top
|
Expression in a distinct domain lateral to the node.
|
Specimen
4I lateral view:
embryonic day 7.5
(more )
Specimen
4I lateral view:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo |
Present |
Regionally restricted |
4I bottom
|
Expression in a distinct domain lateral to the node.
|
Specimen
Not Shown, +/-:
embryonic day 7.5; Dll1tm1Gos/Dll1 +
(more )
Specimen
Not Shown, +/-:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Dll1tm1Gos/Dll1+
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Age:
|
embryonic day 7.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo |
Present |
Regionally restricted |
|
Expression in a distinct domain lateral to the node.
|
Specimen
4K top view:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
Mutant Allele(s):
|
Dll1tm1Gos/Dll1tm1Gos
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: node |
Present |
|
4K top
|
|
TS11: embryo |
Present |
Regionally restricted |
4K top
|
Expression in surrounding tissues was not restricted to the usual domains.
|
Specimen
4K lateral view:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
|
Mutant Allele(s):
|
Dll1tm1Gos/Dll1tm1Gos
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Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo |
Present |
Regionally restricted |
4K bottom
|
Expression in surrounding tissues was not restricted to the usual domains.
|
|