Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:75937
Damert A, et al., Insufficient VEGFA activity in yolk sac endoderm compromises haematopoietic and endothelial differentiation. Development. 2002 Apr;129(8):1881-92
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:2446618
|
|
Gene symbol:
|
Kdr
|
|
Gene name:
|
kinase insert domain protein receptor
|
|
Probe:
|
VEGFR2 probe1
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
VEGFR2 E8.0 +/+:
embryonic day 8.0
(more  )
Specimen
VEGFR2 E8.0 +/+:
(close  )
|
Genetic Background:
|
involves: 129/Sv * CD-1
|
|
Age:
|
embryonic day 8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
VEGFR2 E8.0 -/-:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
|
|
Mutant Allele(s):
|
Vegfatm2.1Nagy/Vegfatm2.1Nagy
|
|
Age:
|
embryonic day 8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
VEGFR2 E9.0 +/+:
embryonic day 9.0
(more )
Specimen
VEGFR2 E9.0 +/+:
(close )
|
Genetic Background:
|
involves: 129/Sv * CD-1
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
VEGFR2 E9.0 -/-:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
|
|
Mutant Allele(s):
|
Vegfatm2.1Nagy/Vegfatm2.1Nagy
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4A:
embryonic day 9.0
(more )
Note: Text and fig. legend disagree on embryo stage. Text states E 8.5 and legend states E9.0. E9.0 was used as stage.
Specimen
4A:
(close )
|
Genetic Background:
|
involves: 129/Sv * CD-1
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Text and fig. legend disagree on embryo stage. Text states E 8.5 and legend states E9.0. E9.0 was used as stage.
|
Specimen
4B:
embryonic day 9.0; Vegfatm2.1Nagy/ Vegfatm2.1Nagy
(more )
Note: Text and fig. legend disagree on embryo stage. Text states E 8.5 and legend states E9.0. E9.0 was used as stage.
Specimen
4B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
|
|
Mutant Allele(s):
|
Vegfatm2.1Nagy/Vegfatm2.1Nagy
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Text and fig. legend disagree on embryo stage. Text states E 8.5 and legend states E9.0. E9.0 was used as stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: cardinal vein |
Weak |
|
4B
|
Expression is much lower than in wild type.
|
| TS14: heart |
Weak |
Regionally restricted |
4B
|
Authors state expression detected in the endocardium and is much lower than in wild type.
|
Specimen
4C:
embryonic day 9.0
(more )
Specimen
4C:
(close )
|
Genetic Background:
|
involves: 129/Sv * CD-1
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: extraembryonic component |
Present |
Regionally restricted |
4C
|
Expression detected in the endothelial lining of blood islands in the yolk sac.
|
Specimen
4D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * CD-1
|
|
Mutant Allele(s):
|
Vegfatm2.1Nagy/Vegfatm2.1Nagy
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: extraembryonic component |
Present |
Regionally restricted |
4D
|
Expression detected in the endothelial lining of blood islands in the yolk sac.
|
|
Analysis Tools