Probe:	Pax6 cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	3A	Transcripts detected over extensive region of head neural ectoderm.
TS12: optic sulcus neural ectoderm	Present		3A	Authors report expression in optic pit.
TS12: future hindbrain floor plate	Absent		3A
TS12: future midbrain floor plate	Absent		3A
TS12: future prosencephalon floor plate	Absent		3A
TS12: surface ectoderm	Present	Regionally restricted	3A	Expression in broad region of head surface ectoderm covering prosencephalon; not hindbrain region or presumptive first branchial arch.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	3B	Segmented expression in presumptive diencephalon, mainly dorsal, reaching neural folds segmented. Weaker in presumptive telencephalon; neural ectoderm expression weak or undetectable at neural folds.
TS13: future prosencephalon neural fold	Present	Regionally restricted	3B	Segmented expression in presumptive diencephalon, mainly dorsal, reaching neural folds segmented. Weaker in presumptive telencephalon; neural ectoderm expression weak or undetectable at neural folds.
TS13: optic pit neural ectoderm	Strong	Regionally restricted	3B	Authors report that expression is strong in optic vesicle,
TS13: surface ectoderm	Present	Regionally restricted	3B	Expression in surface ectoderm is still broad, and extends rostrally to neural folds.

Structure	Level	Pattern	Image	Note
TS15: lens placode	Present	Regionally restricted	3C	Expression in lens placode forms part of a larger domain that extends in dorsal-caudal direction.

Structure	Level	Pattern	Image	Note
TS16: lens pit	Present	Regionally restricted	3D	Expression in surface ectoderm becomes restricted to developing lens placode, nasal placode and immediately adjacent tissue.
TS16: nasal pit	Present	Regionally restricted	3D	Expression in surface ectoderm becomes restricted to developing lens placode, nasal placode and immediately adjacent tissue.
TS16: surface ectoderm	Present	Regionally restricted	3D	Expression in surface ectoderm becomes restricted to developing lens placode, nasal placode and immediately adjacent tissue.

Structure	Level	Pattern	Image	Note
TS16: lens pit	Present	Regionally restricted		Authors report expression in lens and nasal placodes, clearly separated by a region of non-expressing ectoderm.
TS16: nasal pit	Present	Regionally restricted		Authors report expression in lens and nasal placodes, clearly separated by a region of non-expressing ectoderm.
TS16: surface ectoderm	Present	Regionally restricted		Authors report expression in lens and nasal placodes, clearly separated by a region of non-expressing ectoderm.

Structure	Level	Pattern	Image	Note
TS17: eye surface ectoderm	Present	Regionally restricted	3E	As lens pit formation proceeds, expression domain is further restricted until it lies exclusively within developing eye region.
TS17: lens pit	Present	Regionally restricted	3E	As lens pit formation proceeds, expression domain is further restricted until it lies exclusively within developing eye region.

Structure	Level	Pattern	Image	Note
TS23: cornea	Present	Regionally restricted	3F	Expression continues in parts of eye derived from surface ectoderm, including lens pit, lens vesicle and lens, as well as developing cornea.
TS23: eye	Present	Regionally restricted	3F	Expression continues in parts of eye derived from surface ectoderm, including lens pit, lens vesicle and lens, as well as developing cornea.
TS23: lens	Present	Regionally restricted	3F	Expression continues in parts of eye derived from surface ectoderm, including lens pit, lens vesicle and lens, as well as developing cornea.

Structure	Level	Pattern	Image	Note
TS23: eye	Present	Regionally restricted	4C	Authors report expression predominantly in 2 neuroectodermal layers of optic cup-like structure.
TS23: optic stalk	Weak		4C	Little expression in optic stalk.

Structure	Level	Pattern	Image	Note
TS20: eye	Present	Regionally restricted	4D
TS20: pigmented retinal epithelium	Absent		4D	Authors report considerable region of pigmented retinal epithelium that does not express Pax-6.

Structure	Level	Pattern	Image	Note
TS20: eye	Present	Regionally restricted	4E	Authors describe distally restricted expression domains.

Structure	Level	Pattern	Image	Note
TS12: eye	Present	Regionally restricted		Authors report no differences from normal pattern of expression in mutant embryo eye.

Structure	Level	Pattern	Image	Note
TS13: eye	Present		5A

Structure	Level	Pattern	Image	Note
TS13: eye	Present	Regionally restricted	5B	Authors report that homozygous mutant embryos have the same expression pattern in optic vesicles as their +/+ and +/- littermates.
TS13: optic pit	Present	Regionally restricted	5B	Authors report that homozygous mutant embryos have the same expression pattern in optic vesicles as their +/+ and +/- littermates.

Structure	Level	Pattern	Image	Note
TS16: surface ectoderm	Present	Regionally restricted	5C (right)	Expression in surface ectoderm adjacent to optic vesicle; strongest in most distal structures; confined to around lens and nasal placodes.

Structure	Level	Pattern	Image	Note
TS16: surface ectoderm	Absent		5C (left)	No expression in surface ectoderm anywhere in the head, including the eye region; no expression in surface ectoderm adjacent to optic vesicle.

Structure	Level	Pattern	Image	Note
TS15: lens placode	Present		6A	Expression in nasal region. Authors report expression in nasal placode at a lower level than in lens placode.
TS15: nose	Present		6A	Expression in nasal region. Authors report expression in nasal placode at a lower level than in lens placode.
TS15: olfactory placode	Present		6A	Expression in nasal region. Authors report expression in nasal placode at a lower level than in lens placode.

Structure	Level	Pattern	Image	Note
TS17: nasal pit	Present	Regionally restricted	6B	Expression in nasal region; continues in placodal epithelium during formation of nasal pits.
TS17: nose	Present	Regionally restricted	6B	Expression in nasal region; continues in placodal epithelium during formation of nasal pits.

Structure	Level	Pattern	Image	Note
TS17: olfactory epithelium	Present			Expression detectable in developing olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS16: nose	Present	Regionally restricted	6C	Authors report expression detectable in nasal placodes.

Structure	Level	Pattern	Image	Note
TS16: nose	Absent		6D	Authors report absence of expression in nasal placode and nasal region.
TS16: future forebrain	Present		6D	Expression in forebrain neuroepithelium.