Probe:

Sp5 probe

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Uniform	2A

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Uniform	2B

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		2C

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		2D

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present		2E
TS12: future midbrain	Weak		2E
TS12: future prosencephalon	Weak		2E	Expression is in the future diencephalon.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present		2F
TS13: primitive streak	Present		2F
TS13: future prosencephalon	Present	Regionally restricted	2F	Expression is in the future diencephalon.

Structure	Level	Pattern	Image	Note
TS14: primitive streak	Present		2G
TS14: future midbrain	Present	Regionally restricted	2G	Expression is in both the dorsal and lateral regions.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	2H	Expression is in both the dorsal and lateral regions.

Structure	Level	Pattern	Image	Note
TS16: future midbrain	Present	Regionally restricted	2I	Expression is in the dorsal part, but expression in the lateral part is fading.

Structure	Level	Pattern	Image	Note
TS17: tail mesenchyme	Strong		2J
TS17: midbrain	Present	Regionally restricted	2J	There is dorsal expression, but the lateral expression is fading.

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present		3A
TS11: embryo ectoderm	Absent		3A
TS11: extraembryonic visceral endoderm	Absent		3A
TS11: embryo mesoderm	Present	Regionally restricted	3A	There is expression in the ingressing mesoderm, but the lateral mesoderm does not express.

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present	Regionally restricted	3B	Expression is at the posterior end.
TS13: embryo mesenchyme	Present	Regionally restricted	3B	Expression is in the mesoderm underlying the posterior neural plate.

Structure	Level	Pattern	Image	Note
TS15: gut	Present		3C
TS15: notochord	Present		3C

Structure	Level	Pattern	Image	Note
TS17: tail mesenchyme	Strong		3D
TS17: notochord	Present		3D

Structure	Level	Pattern	Image	Note
TS17: tail mesenchyme	Strong		3E
TS17: tail nervous system	Absent		3E	The tail ectoderm is negative.

Structure	Level	Pattern	Image	Note
TS17: notochord	Present	Single cells	3F	Expression is in a single cell surrounded by negative cells.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present		4A
TS12: future prosencephalon	Present	Regionally restricted	4A	Expression is in the region of the diencephalon.

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Very strong	Regionally restricted	4B	Expression is in the dorso-lateral regions.
TS14: future diencephalon	Present	Regionally restricted	4B	Expression is in the prospective pretectum and epithalamus.
TS14: prosencephalon	Present	Regionally restricted	4B	There is no staining in the telencephalon.

Structure	Level	Pattern	Image	Note
TS16: future midbrain	Present	Regionally restricted	4C	The lateral expression is gradually disappearing.

Structure	Level	Pattern	Image	Note
TS16: future midbrain lateral wall	Strong	Regionally restricted	4D	Expression is at the midbrain-hindbrain boundary throughout the dorsal-ventral axis.
TS16: rhombomere 1 lateral wall	Strong	Regionally restricted	4D	Expression is at the midbrain-hindbrain boundary throughout the dorsal-ventral axis.
TS16: future midbrain roof plate	Absent		4D
TS16: rhombomere 1 roof plate	Absent		4D

Structure	Level	Pattern	Image	Note
TS16: diencephalon roof plate	Absent		4E
TS16: diencephalon lateral wall	Present	Regionally restricted	4E	Expression is in the anlage of the epithalamus and pretectum.

Structure	Level	Pattern	Image	Note
TS16: future midbrain roof plate	Absent		4F
TS16: future midbrain lateral wall	Present	Regionally restricted	4F	Expression is in the tegmentum.
TS16: future midbrain floor plate	Present	Regionally restricted	4F	In the entire brain, only the area of the hypothalamus expresses.

Structure	Level	Pattern	Image	Note
TS16: future midbrain	Present	Regionally restricted	4G	Expression is in a stripe at the base of the pretectum and hypothalamus and within the anlage of the mammilary bodies. There is also expression in the tegmentum.

Structure	Level	Pattern	Image	Note
TS16: future midbrain	Present	Regionally restricted	4H	Expression is in a stripe at the base of the pretectum and hypothalamus and within the anlage of the mammilary bodies. There is also expression in the tegmentum.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	4I	Expression is strong in the dorsolateral regions.

Structure	Level	Pattern	Image	Note
TS16: future midbrain floor plate	Present	Regionally restricted	4J	In the entire brain, only the area of the hypothalamus expresses.
TS16: diencephalon floor plate	Absent		4J
TS16: future hindbrain floor plate	Absent		4J
TS16: telencephalon floor plate	Absent		4J

Structure	Level	Pattern	Image	Note
TS16: neural tube lateral wall	Present	Graded	4K	The expression level peaks in the upper toraxic region and fades toward the rhombencephalon and the tail.

Structure	Level	Pattern	Image	Note
TS16: neural tube lateral wall	Present	Regionally restricted	4L	Expression is restricted to the alar plate.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted	4M	Expression is confined to the medial-dorsal part.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Strong	Regionally restricted	4N	Expression is confined to the medio-dorsal part.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Strong	Regionally restricted	4O	Expression is confined to the medio-dorsal part.

Structure	Level	Pattern	Image	Note
TS16: trigeminal ganglion	Weak		4P

Structure	Level	Pattern	Image	Note
TS17: trigeminal ganglion	Present		4Q

Structure	Level	Pattern	Image	Note
TS13: 1st branchial groove ectoderm	Present		4R
TS13: 1st branchial pouch endoderm	Present		4R
TS13: 2nd branchial arch ectoderm	Present		4R
TS13: 2nd branchial pouch endoderm	Present		4R

Structure	Level	Pattern	Image	Note
TS13: 1st branchial groove ectoderm	Present		4S
TS13: 1st branchial pouch endoderm	Present		4S

Structure	Level	Pattern	Image	Note
TS17: 1st branchial groove	Present		4T
TS17: 1st branchial pouch	Present		4T
TS17: 2nd branchial groove	Present		4T
TS17: 2nd branchial pouch	Present		4T
TS17: 3rd branchial groove	Present		4T
TS17: 3rd branchial pouch	Present		4T
TS17: 4th branchial groove	Absent		4T
TS17: 4th branchial pouch	Absent		4T

Structure	Level	Pattern	Image	Note
TS12: head somite	Present	Regionally restricted	4U	About 2 hours after the formation of a somite, expression is in the dorsal tip, which will give rise to the epaxial dermomyotome lip. Expression levels peak after 15 hours and then fade away.
TS12: trunk somite	Present	Regionally restricted	4U	About 2 hours after the formation of a somite, expression is in the dorsal tip, which will give rise to the epaxial dermomyotome lip. Expression levels peak after 15 hours and then fade away.

Structure	Level	Pattern	Image	Note
TS16: tail somite	Present	Regionally restricted	4V	About 2 hours after the formation of a somite, expression is in the dorsal tip, which will give rise to the epaxial dermomyotome lip. Expression levels peak after 15 hours and then fade away.

Structure	Level	Pattern	Image	Note
TS13: head somite	Present	Regionally restricted	4W	About 2 hours after the formation of a somite, expression is in the dorsal tip, which will give rise to the epaxial dermomyotome lip. Expression levels peak after 15 hours and then fade away.
TS13: trunk somite	Present	Regionally restricted	4W	About 2 hours after the formation of a somite, expression is in the dorsal tip, which will give rise to the epaxial dermomyotome lip. Expression levels peak after 15 hours and then fade away.

Structure	Level	Pattern	Image	Note
TS16: tail somite	Present	Regionally restricted	4X	About 2 hours after the formation of a somite, expression is in the dorsal tip, which will give rise to the epaxial dermomyotome lip.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Single cells	4Y