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Gene Expression Data
Assay Details
Assay
Reference: J:77125 Norris DP, et al., The Foxh1-dependent autoregulatory enhancer controls the level of Nodal signals in the mouse embryo. Development. 2002 Jul;129(14):3455-68
Assay type: RNA in situ
MGI Accession ID: MGI:2183531
Gene symbol: Pitx2
Gene name: paired-like homeodomain transcription factor 2
Probe: Pitx2 cDNA
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 6C: embryonic day 8.5 (more )
Note: 3-somite stage.
Structure Level Pattern Image Note
TS13: head mesenchyme Present 6C
TS13: lateral plate mesoderm Present Regionally restricted 6C Expression is in the left lateral plate mesoderm.

Specimen 6D: embryonic day 8.5; Nodaltm2Rob/Nodaltm2Rob (more )
Structure Level Pattern Image Note
TS13: head mesenchyme Present 6D
TS13: lateral plate mesoderm Weak Regionally restricted 6D Expression is in the lateral plate mesoderm and is more posteriorly restricted compared to wild-type embryos.

Specimen 6E: embryonic day 8.75 (more )
Structure Level Pattern Image Note
TS13: head mesenchyme Present 6E
TS13: lateral plate mesoderm Present Regionally restricted 6E Expression in the left lateral plate mesoderm extends behind the sinoatrial regoin of the heart.
TS13: extraembryonic component Present Regionally restricted 6E Expression is in the visceral yolk sac.

Specimen 6F: embryonic day 8.75; Nodaltm2Rob/Nodaltm2Rob (more )
Structure Level Pattern Image Note
TS13: head mesenchyme Present 6F
TS13: lateral plate mesoderm Present Regionally restricted 6F Expression in the left lateral plate mesoderm is posteriorly restricted. Expression is absent from the region behing the sinoatrial region of the heart.
TS13: extraembryonic component Present Regionally restricted 6F Expression is in the visceral yolk sac.

Specimen 6G: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: mesenchyme derived from lateral plate Present Regionally restricted 6G Expression is in the left lateral plate mesoderm.

Specimen 6H: embryonic day 9.0; Nodaltm2Rob/Nodaltm2Rob (more )
Structure Level Pattern Image Note
TS14: mesenchyme derived from lateral plate Present Regionally restricted 6H The expression in the left lateral plate mesoderm is limited to more posterior levels than in wild-type embryos. The shift is approximately the width of two-somites.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory