Probe:	Mab21l2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: future brain	Present	Regionally restricted	1B	Expression at the anterior neural folds.
TS13: future hindbrain	Strong	Regionally restricted	1B	Cephalic expression at the mid-hindbrain region.
TS13: future midbrain	Strong	Regionally restricted	1B	Cephalic expression at the mid-hindbrain region.

Structure	Level	Pattern	Image	Note
TS13: anterior pro-rhombomere neural fold	Strong		1C
TS13: future midbrain neural fold	Strong		1C
TS13: branchial arch	Strong		1C
TS13: optic eminence	Strong		1C	Optic and otic tissues
TS13: otic placode	Strong		1C	Optic and otic tissues

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Strong	Regionally restricted	1D	Midbrain -hindbrain region.
TS14: future midbrain	Strong	Regionally restricted	1D	Midbrain -hindbrain region.
TS14: branchial arch	Strong		1D
TS14: eye	Strong		1D	Optic and otic tissues.
TS14: inner ear	Strong		1D	Optic and otic tissues.
TS14: forelimb bud	Present		1D

Structure	Level	Pattern	Image	Note
TS16: future hindbrain	Strong	Regionally restricted	1E	Midbrain -hindbrain region.
TS16: future midbrain	Strong	Regionally restricted	1E	Midbrain -hindbrain region.
TS16: branchial arch	Strong		1E
TS16: inner ear	Strong		1E	Otic tissues.
TS16: optic cup inner layer	Strong		1E
TS16: forelimb bud	Present		1E

Structure	Level	Pattern	Image	Note
TS18: hindbrain	Strong	Regionally restricted	1F	Midbrain -hindbrain region.
TS18: midbrain	Strong	Regionally restricted	1F	Midbrain -hindbrain region.
TS18: branchial arch	Strong		1F
TS18: inner ear	Strong		1F	Otic tissues.
TS18: retina	Strong		1F
TS18: extraembryonic component	Strong	Regionally restricted	1F	Umbilical cord

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Strong	Regionally restricted	1G	Midbrain-hindbrain region.
TS20: midbrain	Strong	Regionally restricted	1G	Midbrain-hindbrain region.
TS20: oral region	Strong		1G
TS20: inner ear	Strong		1G	Otic tissues.
TS20: retina	Strong		1G
TS20: extraembryonic component	Strong	Regionally restricted	1G	Umbilical cord

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Strong	Regionally restricted	1H	Midbrain-hindbrain region.
TS21: midbrain	Strong	Regionally restricted	1H	Midbrain-hindbrain region.
TS21: oral region	Strong		1H
TS21: inner ear	Strong		1H	Otic tissues.
TS21: retina	Present		1H
TS21: vibrissa	Absent		1H
TS21: pinna	Present	Regionally restricted	1H	Anterior portion of the pinna.
TS21: extraembryonic component	Strong	Regionally restricted	1H	Umbilical cord

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Strong	Regionally restricted	1I	Midbrain-hindbrain region.
TS22: midbrain	Strong	Regionally restricted	1I	Midbrain-hindbrain region.
TS22: oral region	Strong		1I
TS22: inner ear	Strong		1I	Otic tissues.
TS22: retina	Weak		1I
TS22: extraembryonic component	Absent		1I

Structure	Level	Pattern	Image	Note
TS22: lower jaw	Weak
TS22: tongue	Weak
TS22: upper jaw	Weak

Structure	Level	Pattern	Image	Note
TS23: hindbrain	Present	Regionally restricted		Residual expression.
TS23: lower jaw	Absent
TS23: tongue	Absent
TS23: upper jaw	Absent
TS23: cervical vertebra	Absent
TS23: hindlimb interdigital region	Absent

Structure	Level	Pattern	Image	Note
TS23: hindbrain	Present	Regionally restricted		Residual expression.
TS23: lower jaw	Absent
TS23: tongue	Absent
TS23: upper jaw	Absent

Structure	Level	Pattern	Image	Note
TS24: hindbrain	Absent			Absence of expression with the the completion of hindbrain development.
TS24: lower jaw	Absent
TS24: tongue	Absent
TS24: upper jaw	Absent

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	2A	Expression at the dorsal midline.
TS16: forelimb bud	Present	Regionally restricted	2A	Limb buds and digits.
TS16: hindlimb bud	Present	Regionally restricted	2A	Limb buds and digits.
TS16: trunk somite	Present		2A	In early and differentiating somites.

Structure	Level	Pattern	Image	Note
TS18: neural tube	Present	Regionally restricted	2B	Expression at the dorsal midline.
TS18: forelimb bud	Present		2B	Limb buds and digits.
TS18: hindlimb bud	Present		2B	Limb buds and digits.
TS18: tail somite	Present		2B	In early and differentiating somites.
TS18: trunk somite	Present		2B	In early and differentiating somites.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	2C	Expression at the dorsal midline.
TS20: forelimb	Present		2C
TS20: forelimb digit	Present		2C
TS20: hindlimb	Present		2C
TS20: hindlimb digit	Present		2C
TS20: tail somite	Present		2C	In early and differentiating somites.
TS20: trunk mesenchyme	Present	Regionally restricted	2C	In early and differentiating somites.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	2D	Expression at the dorsal midline.
TS21: tail spinal cord	Present	Regionally restricted	2D	Expression at the dorsal midline.
TS21: forelimb	Present		2D
TS21: forelimb digit	Present		2D
TS21: hindlimb	Present		2D
TS21: hindlimb digit	Present		2D
TS21: tail somite	Present		2D	In early and differentiating somites.
TS21: trunk mesenchyme	Present	Regionally restricted	2D	In early and differentiating somites.
TS21: thoracic vertebral cartilage condensation	Present		2D

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	2E	Expression at the dorsal midline.
TS22: tail spinal cord	Present	Regionally restricted	2E	Expression at the dorsal midline.
TS22: forelimb	Present		2E
TS22: hindlimb	Present		2E
TS22: tail somite	Present		2E	In early and differentiating somites.
TS22: trunk mesenchyme	Present	Regionally restricted	2E	In early and differentiating somites.
TS22: cervical vertebral cartilage condensation	Present		2E

Structure	Level	Pattern	Image	Note
TS16: hindlimb bud	Present	Regionally restricted	3A

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud	Present	Regionally restricted	3B

Structure	Level	Pattern	Image	Note
TS20: hindlimb interdigital region	Present		3C

Structure	Level	Pattern	Image	Note
TS21: hindlimb interdigital region	Present	Regionally restricted	3D

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present	Regionally restricted	3E
TS22: hindlimb	Present	Regionally restricted	3E
TS22: hindlimb interdigital region	Weak		3E

Structure	Level	Pattern	Image	Note
TS18: mesencephalic vesicle	Present	Regionally restricted	4A	Expression in the epithelium overlying the mesencephalic vesicle.
TS18: 4th ventricle	Present	Regionally restricted	4A	Expression in the caudal extremity of the 4th ventricle.

Structure	Level	Pattern	Image	Note
TS18: neural tube	Present	Regionally restricted	4B	Expression in the dorsolateral tissues.
TS18: trunk dermomyotome	Present		4B

Structure	Level	Pattern	Image	Note
TS18: tail dermomyotome	Present		4C
TS18: trunk dermomyotome	Present		4C
TS18: extraembryonic component	Present	Regionally restricted	4C	Umbilical cord outer layer (epithelial tissue).

Structure	Level	Pattern	Image	Note
TS20: retina	Present		4D