Gene Expression Data
Assay Details
Assay
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Reference:
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J:74631
Tian E, et al., Otx2 is required to respond to signals from anterior neural ridge for forebrain specification. Dev Biol. 2002 Feb 15;242(2):204-23
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2179002
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Gene symbol:
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Emx2
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Gene name:
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empty spiracles homeobox 2
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Probe:
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Emx2 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4C:
embryonic day 9.5
(more )
Specimen
4C:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4D:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx2tm1Sia/Otx2tm4Sia
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5A:
embryonic day 8.5
(more )
Specimen
5A:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS14: prosencephalon |
Present |
Regionally restricted |
5A
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Expression in prospective telencephalon and dorsal diencephalon.
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Specimen
5B:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx2tm1Sia/Otx2tm4Sia
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
not shown:
embryonic day 8.0
(more )
Note: 3 somite stage.
Specimen
not shown:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
|
embryonic day 8.0
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Age Note:
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Age and stage of embryo determined from somite number.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3 somite stage.
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Structure |
Level |
Pattern |
Image |
Note |
TS12: neural ectoderm |
Present |
Regionally restricted |
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Authors report expression is initiated in the laterocaudal neural plate at the 3-somite stage.
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Specimen
8G:
embryonic day 8.5
(more )
Note: 8 somite stage.
Specimen
8G:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.5
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Age Note:
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Age and stage of embryo determined from somite number.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8 somite stage.
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Specimen
8H:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx2tm1Sia/Otx2tm4Sia
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Age:
|
embryonic day 8.5
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Age Note:
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Age and stage of embryo determined from somite number.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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8 somite stage.
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