Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75954
Dunwoodie SL, et al., Axial skeletal defects caused by mutation in the spondylocostal dysplasia/pudgy gene Dll3 are associated with disruption of the segmentation clock within the presomitic mesoderm. Development. 2002 Apr;129(7):1795-806
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2178636
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Gene symbol:
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Uncx
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Gene name:
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UNC homeobox
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Probe:
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Uncx4.1 cDNA4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6C:
embryonic day 9.5; Dll3tm1Rbe/Dll3 +
(more  )
Specimen
6C:
(close  )
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Genetic Background:
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involves: 129P2/Ola * C57BL/6
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Mutant Allele(s):
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Dll3tm1Rbe/Dll3+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Bouin's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: trunk somite |
Present |
Regionally restricted |
6C
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Authors report that expression shows clear periodicity. Expression marks the posterior nascent (most recently formed) somites.
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Specimen
6F:
embryonic day 9.5; Dll3tm1Rbe/Dll3 +
(more )
Specimen
6F:
(close )
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Genetic Background:
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involves: 129P2/Ola * C57BL/6
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Mutant Allele(s):
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Dll3tm1Rbe/Dll3+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Bouin's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk somite |
Present |
Regionally restricted |
6F
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In somites dissected from specimen 6C, expression is clearly restricted to the posterior of epithelial somites.
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Specimen
6D:
(close )
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Genetic Background:
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involves: 129P2/Ola * C57BL/6
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Mutant Allele(s):
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Dll3tm1Rbe/Dll3tm1Rbe
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Bouin's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk paraxial mesenchyme |
Present |
Regionally restricted |
6D
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Authors report expression is reduced; in this embryo, periodic expression is partially retained.
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Specimen
6G:
(close )
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Genetic Background:
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involves: 129P2/Ola * C57BL/6
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Mutant Allele(s):
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Dll3tm1Rbe/Dll3tm1Rbe
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Bouin's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk paraxial mesenchyme |
Present |
Regionally restricted |
6G
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In trunk paraxial mesoderm dissected from specimen 6D, expression appears periodic but is not restricted to the posterior of somites.
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Specimen
6E:
(close )
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Genetic Background:
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involves: 129P2/Ola * C57BL/6
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Mutant Allele(s):
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Dll3tm1Rbe/Dll3tm1Rbe
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Bouin's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk paraxial mesenchyme |
Present |
Regionally restricted |
6E
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Authors report expression is reduced; in this embryo, periodic expression is lost.
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Analysis Tools