Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75942
Ybot-Gonzalez P, et al., Sonic hedgehog and the molecular regulation of mouse neural tube closure. Development. 2002 May;129(10):2507-17
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2178347
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Gene symbol:
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Ptch1
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Gene name:
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patched 1
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Probe:
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M21-3'UTR
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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The authors developed their own staging system based on the three morphological patterns of neural plate bending: mode 1 embryos have 7-15 somites (E8.5); mode 2 embryos have 16-24 somites (early E9.5); and mode 3 embryos have 25-30 somites (E10).
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Results
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Specimen
1M:
embryonic day 8.5
(more  )
Specimen
1M:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future spinal cord neural plate |
Very strong |
Regionally restricted |
1M
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There is intense staining at the ventral midline of the plate (beneath the posterior neuropore). There is no staining in the dorsal neural plate.
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Specimen
1N:
embryonic day 9.5
(more )
Specimen
1N:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future spinal cord neural plate |
Moderate |
Regionally restricted |
1N
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There is staining at the ventral midline of the plate (beneath the posterior neuropore), but its intensity is reduced from earlier in development. There is no staining in the dorsal neural plate.
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Specimen
1O:
embryonic day 10.0
(more )
Specimen
1O:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Weak |
Regionally restricted |
1O
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There is staining at the ventral midline of the plate (beneath the posterior neuropore), but it is of relatively low intensity compared to earlier developmental times. There is no staining in the dorsal neural plate.
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