Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:75942
Ybot-Gonzalez P, et al., Sonic hedgehog and the molecular regulation of mouse neural tube closure. Development. 2002 May;129(10):2507-17
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:2178345
|
|
Gene symbol:
|
Shh
|
|
Gene name:
|
sonic hedgehog
|
|
Probe:
|
Shh cDNA2
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Assay notes:
|
The authors developed their own staging system based on the three morphological patterns of neural plate bending: mode 1 embryos have 7-15 somites (E8.5); mode 2 embryos have 16-24 somites (early E9.5); and mode 3 embryos have 25-30 somites (E10).
|
|
|
Results
|
Specimen
1A:
embryonic day 8.5
(more  )
Note: This specimen had 10 somites.
Specimen
1A:
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
This specimen had 10 somites.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: notochord |
Present |
Graded |
1A
|
There is staining along the entire length of the notochord, but its intensity under the posterior neuropore varies according to developmental stage.
|
Specimen
1B:
embryonic day 9.5
(more )
Note: This specimen had 22 somites.
Specimen
1B:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
This specimen had 22 somites.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: notochord |
Present |
Graded |
1B
|
There is staining along the entire length of the notochord, but its intensity under the posterior neuropore varies according to developmental stage.
|
Specimen
1C:
embryonic day 10.0
(more )
Note: This specimen had 28 somites.
Specimen
1C:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
This specimen had 28 somites.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: notochord |
Present |
Graded |
1C
|
There is staining along the entire length of the notochord, but its intensity under the posterior neuropore varies according to developmental stage.
|
Specimen
1D:
embryonic day 8.5
(more )
Specimen
1D:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future spinal cord neural plate |
Present |
Graded |
1D
|
There is staining along the entire length of the notochord, but its intensity under the posterior neuropore varies according to developmental stage.
|
Specimen
1E:
embryonic day 9.5
(more )
Specimen
1E:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: notochord |
Present |
Graded |
1E
|
There is staining along the entire length of the notochord, but its intensity under the posterior neuropore varies according to developmental stage.
|
Specimen
1F:
embryonic day 10.0
(more )
Specimen
1F:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: notochord |
Present |
Graded |
1F
|
There is staining along the entire length of the notochord, but its intensity under the posterior neuropore varies according to developmental stage.
|
Specimen
1J:
embryonic day 8.5
(more )
Note: This was a vibratome section of a whole-mount stained embryo.
Specimen
1J:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Note:
|
This was a vibratome section of a whole-mount stained embryo.
|
Specimen
1K:
embryonic day 9.5
(more )
Note: This was a vibratome section of a whole-mount stained embryo.
Specimen
1K:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Note:
|
This was a vibratome section of a whole-mount stained embryo.
|
Specimen
1L:
embryonic day 10.0
(more )
Note: This was a vibratome section of a whole-mount stained embryo.
Specimen
1L:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Note:
|
This was a vibratome section of a whole-mount stained embryo.
|
|
Analysis Tools