Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:73506
Lowe LA, et al., Genetic dissection of nodal function in patterning the mouse embryo. Development. 2001 May;128(10):1831-43
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:2159412
|
|
Gene symbol:
|
T
|
|
Gene name:
|
brachyury, T-box transcription factor T
|
|
Probe:
|
brachyury probe14
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
3. A.:
embryonic day 7.0
(more  )
Specimen
3. A.:
(close  )
|
Genetic Background:
|
involves: 129 * C57BL/6J
|
|
Age:
|
embryonic day 7.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3. B.:
(close )
|
Genetic Background:
|
either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
|
|
Mutant Allele(s):
|
Nodaltm1Mku/Nodaltm1.1Mku
|
|
Age:
|
embryonic day 7.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: primitive streak |
Present |
Regionally restricted |
3B
|
Expression is detected in the proximal streak in a much shorter domain then the wild type.
|
Specimen
3. C.:
embryonic day 7.5
(more )
Specimen
3. C.:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6J
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3. D.:
(close )
|
Genetic Background:
|
either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
|
|
Mutant Allele(s):
|
Nodaltm1Mku/Nodaltm1.1Mku
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: primitive streak |
Present |
Regionally restricted |
3D
|
Expression in the streak is truncated, shorter and more proximal than in wild-type.
|
| TS11: node |
Present |
|
3D
|
|
Specimen
3. E.:
embryonic day 7.5
(more )
Specimen
3. E.:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6J
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
3. F.:
(close )
|
Genetic Background:
|
either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
|
|
Mutant Allele(s):
|
Nodaltm1Mku/Nodaltm1.1Mku
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
Regionally restricted |
3F
|
Expression is detected in the ventral node and the node is in a more proximal location than in wild-type.
|
Specimen
3. G., I.:
embryonic day 8.0
(more )
Note: Specimen 3. I. is a ventral view of 3. G.
Specimen
3. G., I.:
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6J
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Theiler stage of this specimen assigned by curators from EMAGE. Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Specimen 3. I. is a ventral view of 3. G.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: notochordal plate |
Present |
Regionally restricted |
3I
,
3G
|
Expression is detected in the streak, node, notochord and midline.
|
| TS11: notochordal process |
Present |
Regionally restricted |
3I
,
3G
|
Expression is detected in the streak, node, notochord and midline.
|
| TS11: primitive streak |
Present |
Regionally restricted |
3I
,
3G
|
Expression is detected in the streak, node, notochord and midline.
|
Specimen
3. H., J.:
embryonic day 8.0; Nodaltm1Mku/ Nodaltm1.1Mku
(more )
Note: Specimen 3. J. is a ventral view of 3. H.
Specimen
3. H., J.:
(close )
|
Genetic Background:
|
either: (involves: 129S1/Sv * FVB/N) or (involves: 129S1/Sv * C57BL/6J * FVB/N)
|
|
Mutant Allele(s):
|
Nodaltm1Mku/Nodaltm1.1Mku
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Specimen 3. J. is a ventral view of 3. H.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: primitive streak |
Present |
Regionally restricted |
3H
,
3J
|
Expression is detected in the streak and midline but is shorter and more proximal in the streak and reduced and discontinuous in the midline compared to wild-type.
|
|
Analysis Tools