Gene Expression Data
Assay Details
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Assay
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Reference:
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J:71998
Tremblay KD, et al., Mouse embryos lacking Smad1 signals display defects in extra-embryonic tissues and germ cell formation. Development. 2001 Sep;128(18):3609-21
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2155932
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Gene symbol:
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Afp
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Gene name:
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alpha fetoprotein
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Probe:
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AFP probe4
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Authors generated two Madh1 targeted mutations, Madh1<tm1Rob> and Madh1<tm2.1Rob>. Phenotype is stated as identical, but the mutation used in the in-situ experiments is not specified.
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Results
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Specimen
5. A. +/+:
embryonic day 7.5
(more  )
Specimen
5. A. +/+:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5. A. -/-:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129S4/SvJae
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Mutant Allele(s):
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Smad1tm2.1Rob/Smad1tm2.1Rob
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: extraembryonic visceral endoderm |
Present |
Regionally restricted |
5A -/-
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Expression is detected throughout the visceral endoderm (VE) overlying the embryonic region but is excluded from the extra-embryonic region and the anterior aspects of the VE.
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| TS11: trophectoderm |
Absent |
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5A -/-
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Expression is absent from the extra-embryonic ectoderm.
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Specimen
5. A'. -/-:
(close )
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Genetic Background:
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involves: 129S/SvEv * 129S4/SvJae
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Mutant Allele(s):
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Smad1tm2.1Rob/Smad1tm2.1Rob
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools