Gene Expression Data
Assay Details
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Assay
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Reference:
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J:72725
Martinez-Barbera JP, et al., Regionalisation of anterior neuroectoderm and its competence in responding to forebrain and midbrain inducing activities depend on mutual antagonism between OTX2 and GBX2. Development. 2001 Dec;128(23):4789-800
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2155571
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Probe:
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En-1 cDNA5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2E:
embryonic day 8.7
(more  )
Specimen
2E:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future hindbrain |
Present |
Regionally restricted |
2E
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Expression is at the midbrain-hindbrain boundary.
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| TS14: future midbrain |
Present |
Regionally restricted |
2E
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Expression is at the midbrain-hindbrain boundary.
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Specimen
2S:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future brain |
Present |
Regionally restricted |
2S
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There is a caudalization of expression.
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Specimen
4E:
embryonic day 9.7
(more )
Specimen
4E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Age:
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embryonic day 9.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
4J:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx1tm1Asim/Otx1+
Otx2tm1Pas/Otx2+
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Age:
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embryonic day 9.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future brain |
Present |
Regionally restricted |
4J
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The expression domain in this mutant is expanded anteriorly into the dorsal midbrain.
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Analysis Tools