Gene Expression Data
Assay Details
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Assay
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Reference:
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J:72725
Martinez-Barbera JP, et al., Regionalisation of anterior neuroectoderm and its competence in responding to forebrain and midbrain inducing activities depend on mutual antagonism between OTX2 and GBX2. Development. 2001 Dec;128(23):4789-800
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2155564
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Gene symbol:
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Fgf8
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Gene name:
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fibroblast growth factor 8
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Probe:
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Fgf8 cDNA5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2C:
embryonic day 8.7
(more  )
Specimen
2C:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future hindbrain |
Present |
Regionally restricted |
2C
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Expression is at the midbrain-hindbrain boundary.
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| TS13: future midbrain |
Present |
Regionally restricted |
2C
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Expression is at the midbrain-hindbrain boundary.
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Specimen
2Q:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future brain |
Present |
Regionally restricted |
2Q
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The expression domain is caudalized in this mutant.
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Specimen
4C:
embryonic day 9.7
(more )
Specimen
4C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Age:
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embryonic day 9.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
4C
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Expression is on the metencephalic side of the midbrain-hindbrain boundary.
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Specimen
4H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6 * DBA/2
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Mutant Allele(s):
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Otx1tm1Asim/Otx1+
Otx2tm1Pas/Otx2+
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Age:
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embryonic day 9.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
4H
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The domain of expression is expanded broadly into most of the dorsal midbrain.
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| TS15: future midbrain |
Present |
Regionally restricted |
4H
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The domain of expression is expanded broadly into most of the dorsal midbrain.
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Specimen
5K:
embryonic day 8.0
(more )
Note: 6 somite stage.
Specimen
5K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
5K
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Expression is in the midbrain-hindbrain border.
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| TS12: future midbrain |
Present |
Regionally restricted |
5K
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Expression is in the midbrain-hindbrain border.
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Specimen
5K'':
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
5K''
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The expression domain is broader than in wild-type embryos. The expression extends to the posterior border of Otx2 expression.
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Specimen
7C:
embryonic day 8.7
(more )
Specimen
7C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
7M:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 8.7
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Analysis Tools