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Gene Expression Data
Assay Details
Assay
Reference: J:71879 Bernier G, et al., Isolation and characterization of a downstream target of Pax6 in the mammalian retinal primordium. Development. 2001 Oct;128(20):3987-94
Assay type: RNA in situ
MGI Accession ID: MGI:2153749
Gene symbol: Necab2
Gene name: N-terminal EF-hand calcium binding protein 2
Probe: Necab cDNA
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 2A: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: optic pit Present 2A Expression in the optic sulcus and optic vesicles.
TS13: future midbrain roof plate Present 2A Expression in the pre-tectum.
Specimen 2B: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: optic vesicle Present 2B Expression in the optic sulcus and optic vesicles.
TS15: future midbrain Present Regionally restricted 2B Expression spreads at the midline position in the roof of the midbrain. There is also expression in the pre-tectum.
Specimen 2C, D: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: optic vesicle Present 2C , 2D
TS15: future midbrain Present 2D , 2C Expression in the pre-tectum.
Specimen 2E: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: prosencephalon Present 2E Necab
TS14: optic vesicle Present 2E Necab Expression resembled that of Pax6 (see assay MGI:2153750).
TS14: future midbrain Present 2E Necab Expression in the pre-tectum. Expression resembled that of Pax6 (see assay MGI:2153750).
Specimen 2F: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 2F Necab Expression spreads at the midline position in the roof of the midbrain.
Specimen 2G: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: optic vesicle Present Regionally restricted 2G
TS15: optic cup Present Regionally restricted 2G Strong expression in the ciliary margin region.
Specimen 2K: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: pigmented retinal epithelium Present 2K
TS20: neural retinal epithelium Present Regionally restricted 2K Predominant expression in the ciliary margin of the neural retina.
Specimen 4F, +/+: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: optic vesicle Present 4F +/+
TS15: future midbrain Present 4F +/+ Expression in the pre-tectum.
Specimen 4F, -/-: embryonic day 9.5; Pax6tm1Pgr/Pax6tm1Pgr (more )
Structure Level Pattern Image Note
TS15: optic vesicle Absent 4F -/-
TS15: future midbrain Absent 4F -/- Absence of expression in the pre-tectum.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory