Probe:	Hfh2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS16: migrating neural crest	Present		2A	Expression in neural crest cells emigrating from the dorsal neural tube along its entire length from midbrain/hindbrain border to the tail.
TS16: neural crest	Present	Regionally restricted	2A	Expression in premigratory crest cells.

Structure	Level	Pattern	Image	Note
TS16: tail neural crest	Present	Regionally restricted	2B	Expression in dorsal part of caudal neural tube.
TS16: dorsal root ganglion	Present		2B	Expression persists in dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS16: neural crest	Present	Regionally restricted	2E	Expression in cranial neural crest, at hindbrain levels in cells that contribute to the V, VII and IX cranial ganglia.
TS16: neural tube	Present	Regionally restricted	2E	No expression in caudal neural tube/folds; no expression in caudal regions where dorsal root ganglia and sympathetic ganglia are missing.
TS16: tail neural crest	Absent		2E

Structure	Level	Pattern	Image	Note
TS16: tail neural plate	Absent		2F	No expression in the open neural tube.