Probe:	Ptx1 cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Authors state that most figures presented were obtained using this probe; labelling was identical with the 3' probe (Ptx1 cDNA4; MGI:2150937). Probes were alkaline hydrolyzed to an average length of 500 bp.

Structure	Level	Pattern	Image	Note
TS12: stomatodeum	Strong		1A/B	Expression in stomodeal epithelium.
TS12: foregut diverticulum endoderm	Weak	Regionally restricted	1A/B	Weak expression in a patch of rostroventral foregut endoderm, where the ectoderm and endoderm layers meet.

Structure	Level	Pattern	Image	Note
TS13: stomatodeum	Present	Regionally restricted	1C/D	Expression abruptly stops where the epithelium becomes more squamous.
TS13: foregut diverticulum endoderm	Present	Regionally restricted	1C/D	Expression in rostral foregut endoderm, which will develop into oesophageal epithelium.

Structure	Level	Pattern	Image	Note
TS14: pharyngeal region epithelium	Weak	Regionally restricted	1E/F	Weaker labelling of the foregut endoderm is limited to a few cuboidal cells of the ventral wall that are centered around the buccopharyngeal membrane.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component	Present	Regionally restricted	2A/B	Expression in mandibular and maxillary epithelia.
TS15: 1st branchial arch maxillary component	Present	Regionally restricted	2A/B	Expression in mandibular and maxillary epithelia.
TS15: 1st branchial arch mandibular component mesenchyme	Present	Regionally restricted	2A/B	Expression in a central stripe of mandibular mesenchyme; in a proximal-to-distal gradient.
TS15: 1st branchial arch maxillary component mesenchyme	Absent		2A/B
TS15: 2nd branchial arch	Absent		2A/B
TS15: 3rd branchial arch	Absent		2A/B

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	2D/E	The epithelium of the mandibular arch is only labelled in its inner half.
TS17: 1st branchial arch maxillary component	Present	Regionally restricted	2D/E	The epithelium of the maxillary component is labelled; signal decreases toward the midline.
TS17: 1st branchial arch maxillary component mesenchyme	Absent		2D/E	No other labelling in the mesenchyme of the maxillary component or in other branchial arches.
TS17: 2nd branchial arch	Absent		2D/E	No other labelling in the mesenchyme of the maxillary component or in other branchial arches.
TS17: 3rd branchial arch	Absent		2D/E	No other labelling in the mesenchyme of the maxillary component or in other branchial arches.
TS17: 4th branchial arch	Absent		2D/E	No other labelling in the mesenchyme of the maxillary component or in other branchial arches.
TS17: oral epithelium	Strong	Regionally restricted	2D/E	Signal is restricted to tissues of stomodeal origin; ends precisely at the junction between ectoderm and endoderm.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present		2F/G
TS17: nasal pit	Present		2F/G

Structure	Level	Pattern	Image	Note
TS21: adenohypophysis	Present		3A/B	Expression in all epithelia that derive from the stomodeum.
TS21: nasopharynx epithelium	Present		3A/B	Expression in all epithelia that derive from the stomodeum.
TS21: olfactory epithelium	Present		3A/B	Expression in all epithelia that derive from the stomodeum.
TS21: primary palate epithelium	Present		3A/B	Expression in all epithelia that derive from the stomodeum.
TS21: submandibular gland primordium epithelium	Present		3A/B	Expression in all epithelia that derive from the stomodeum.
TS21: tongue epithelium	Present		3A/B	Expression in all epithelia that derive from the stomodeum.
TS21: tooth epithelium	Present	Regionally restricted	3A/B	Expression in dental epithelium.
TS21: tooth mesenchyme	Absent		3A/B
TS21: nasal cavity respiratory epithelium	Absent		3A/B
TS21: Meckel's cartilage	Present	Regionally restricted	3A/B	Expression in derivatives of first arch mesenchyme.
TS21: lower jaw mesenchyme	Present	Regionally restricted	3A/B	Expression in derivatives of first arch mesenchyme.
TS21: tongue extrinsic pre-muscle mass	Present	Regionally restricted	3A/B	Expression in derivatives of first arch mesenchyme.
TS21: tongue	Present	Regionally restricted	3A/B	Expression in the distal portion of the tongue proper.

Structure	Level	Pattern	Image	Note
TS23: adenohypophysis	Present		3C/D	Expression in all epithelia that derive from the stomodeum.
TS23: nasopharynx epithelium	Present		3C/D	Expression in all epithelia that derive from the stomodeum.
TS23: olfactory epithelium	Present		3C/D	Expression in all epithelia that derive from the stomodeum.
TS23: primary palate epithelium	Present		3C/D	Expression in all epithelia that derive from the stomodeum.
TS23: submandibular gland primordium epithelium	Present		3C/D	Expression in all epithelia that derive from the stomodeum.
TS23: tongue epithelium	Present		3C/D	Expression in all epithelia that derive from the stomodeum.
TS23: tooth epithelium	Present	Regionally restricted	3C/D	Expression in dental epithelium; tooth buds are not labelled.
TS23: esophagus epithelium	Present		3C/D
TS23: tongue extrinsic muscle	Present	Regionally restricted	3C/D	Expression in derivatives of first arch mesenchyme.
TS23: tongue	Present	Regionally restricted	3C/D	Expression in the distal portion of the tongue proper. Authors note stripes of expression in the tongue.

Structure	Level	Pattern	Image	Note
TS19: mandibular process mesenchyme	Present	Regionally restricted	4B	Labelling in the lateral part of the mandibular component of the 1st arch.

Structure	Level	Pattern	Image	Note
TS21: esophagus epithelium	Present		4G
TS21: nasal capsule	Present		4G
TS21: olfactory epithelium	Present		4G
TS21: tongue epithelium	Present	Regionally restricted	4G	Labelling of the ventral/distal region.

Structure	Level	Pattern	Image	Note
TS10: extraembryonic component	Present	Regionally restricted	5A	Expression in the posterior extraembryonic mesoderm; first detected in the newly formed mesoderm at the posterior margin of the embryonic ectoderm where the primitive streak initially appears.

Structure	Level	Pattern	Image	Note
TS11: allantois mesoderm	Present	Regionally restricted	5B/C	Expression in allantois, but not at the anterior level (headfold).

Structure	Level	Pattern	Image	Note
TS11: allantois mesoderm	Strong	Regionally restricted	5D/E	At the level of the extraembryonic and embryonic junction, base of allantois is strongly labelled; labelled mesoderm joins in the midline.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	5F/G	Labelling restricted to posterior lateral plate mesoderm; absent from primitive streak and paraxial mesoderm.

Structure	Level	Pattern	Image	Note
TS13: somatopleure	Present		6A/B
TS13: foregut diverticulum endoderm	Ambiguous		6A/B

Structure	Level	Pattern	Image	Note
TS13: somatopleure	Present	Regionally restricted		Expression extends up to the posterior intestinal portal
TS13: splanchnopleure	Present	Regionally restricted		Expression extends up to the posterior intestinal portal

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from somatopleure	Present		6C/D	Expression in the posterior region of the embryo is specific to the lateral plate mesoderm; expression in the thickened somatic mesoderm (body wall) and splanchnic mesoderm .
TS15: mesenchyme derived from splanchnopleure	Present		6C/D	Expression in the posterior region of the embryo is specific to the lateral plate mesoderm; expression in the thickened somatic mesoderm (body wall) and splanchnic mesoderm .
TS15: umbilical artery	Present		6C/D	Authors report expression in umbilical vessels.
TS15: umbilical vein	Present		6C/D	Authors report expression in umbilical vessels.
TS15: umbilical artery extraembryonic part	Present		6C/D	Authors report expression in umbilical vessels.
TS15: umbilical vein extraembryonic part	Present		6C/D	Authors report expression in umbilical vessels.
TS15: hindgut epithelium	Absent		6C/D	No labelling in hindgut endoderm.
TS15: embryo ectoderm	Absent		6C/D	In the posterior end of the embryo, no labelling in ectoderm, endoderm, notochord, somites or nephrogenic duct.
TS15: nephric duct	Absent		6C/D	In the posterior end of the embryo, no labelling in ectoderm, endoderm, notochord, somites or nephrogenic duct.
TS15: notochord	Absent		6C/D	In the posterior end of the embryo, no labelling in ectoderm, endoderm, notochord, somites or nephrogenic duct.
TS15: trunk somite	Absent		6C/D	In the posterior end of the embryo, no labelling in ectoderm, endoderm, notochord, somites or nephrogenic duct.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Absent		6E/F

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Absent			Other sections through forelimbs never showed labelling.

Structure	Level	Pattern	Image	Note
TS19: footplate mesenchyme	Present	Regionally restricted	6G/H	Expression in hindlimbs mainly confined to distal undifferentiated mesenchyme.
TS19: hindlimb bud	Present	Regionally restricted	6G/H	Expression in hindlimbs mainly confined to distal undifferentiated mesenchyme.
TS19: body wall mesenchyme	Present	Regionally restricted	6G/H	Labelling in body wall adjacent to hindlimb bud.

Structure	Level	Pattern	Image	Note
TS19: body wall mesenchyme	Strong	Regionally restricted	7A/B	Hindlimb buds and posterior body wall are strongly labelled.
TS19: hindlimb bud	Strong	Regionally restricted	7A/B	Hindlimb buds and posterior body wall are strongly labelled.
TS19: olfactory epithelium	Present	Regionally restricted	7A/B	Labelling in nasal pit is restricted to the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS19: trunk mesenchyme	Present	Regionally restricted	7C/D	Domain of expression in lateral plate mesoderm extends from umbilical stalk up to, but not inclusive or, forelimb buds.
TS19: maxillary process mesenchyme	Absent		7C/D	Expression not detected in mesenchyme of the maxillary arch.

Structure	Level	Pattern	Image	Note
TS23: bladder	Present	Regionally restricted	7E/F	Expression in derivatives of posterior lateral plate mesoderm.
TS23: genital tubercle of male	Present	Regionally restricted	7E/F	Expression in derivatives of posterior lateral plate mesoderm.
TS23: hindgut	Present	Regionally restricted	7E/F	Expression in derivatives of posterior lateral plate mesoderm.
TS23: pectoral girdle and thoracic body wall musculature	Present	Regionally restricted	7E/F	Expression in derivatives of posterior lateral plate mesoderm.
TS23: glans of male genital tubercle	Present		7E/F
TS23: hindlimb	Present		7E/F
TS23: stomach	Present	Regionally restricted	7E/F	Expression was detected in the epithelium. Expression was detected in derivatives of posterior lateral plate mesoderm.
TS23: adrenal gland	Absent		7E/F
TS23: liver	Absent		7E/F
TS23: metanephros	Absent		7E/F
TS23: pancreas	Absent		7E/F
TS23: intestine epithelium	Present	Regionally restricted	7E/F	Expression was detected rostrally from the midgut level.