Probe:	lambda-mE2.1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probe length was reduced to an average of 150 nucleotides by limited alkaline hydrolysis.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	1A CRABPII	Transcripts detected in neural epithelium, cranial and spinal ganglia.
TS13: future midbrain	Present		1A CRABPII
TS13: future prosencephalon	Present		1A CRABPII
TS13: head mesenchyme	Present		1A CRABPII	Diffuse labelling in the cranial mesenchyme.
TS13: trunk unsegmented mesenchyme	Present	Regionally restricted	1A CRABPII	More intense signal in undifferentiated presomitic mesenchyme.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	1B CRABPII	Transcripts detected in neural epithelium, cranial and spinal ganglia.
TS13: future hindbrain	Present		1B CRABPII
TS13: head mesenchyme	Present		1B CRABPII	Diffuse labelling in the cranial mesenchyme.
TS13: trunk unsegmented mesenchyme	Present	Regionally restricted	1B CRABPII	More intense signal in undifferentiated presomitic mesenchyme.

Structure	Level	Pattern	Image	Note
TS13: branchial arch	Present	Regionally restricted	1C CRABPII	Present in the ventral endoderm of pharyngeal pouches. Intense labelling of pharyngeal arch mesenchyme.
TS13: neural ectoderm	Present	Regionally restricted	1C CRABPII	Present in neural epithelium; particularly abundant in hindbrain and trunk regions. Also present in spinal ganglia.
TS13: future hindbrain	Present		1C CRABPII
TS13: future prosencephalon	Present		1C CRABPII
TS13: neural tube	Present	Uniform	1C CRABPII
TS13: head mesenchyme	Present	Regionally restricted	1C CRABPII	Intense labellling of mesenchyme of frontonasal region.
TS13: migrating neural crest	Absent		1C CRABPII
TS13: head mesenchyme derived from neural crest	Present	Regionally restricted	1C CRABPII	High levels in neural crest cell-derived cranial ganglia.
TS13: trigeminal neural crest	Present		1C CRABPII
TS13: somatopleure	Present		1C CRABPII
TS13: splanchnopleure	Absent		1C CRABPII
TS13: foregut diverticulum endoderm	Present	Regionally restricted	1C CRABPII	Present in ventral endoderm of foregut and midgut.
TS13: midgut endoderm	Present	Regionally restricted	1C CRABPII	Present in ventral endoderm of foregut and midgut.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component mesenchyme	Strong		1D CRABPII
TS15: fronto-nasal process mesenchyme	Strong		1D CRABPII
TS15: head mesenchyme derived from neural crest	Strong	Regionally restricted	1D CRABPII	Intense labelling in subpopulations of neural crest cells that later form the cranial ganglia.
TS15: trunk mesenchyme	Present	Regionally restricted	1D CRABPII	Present in lateral mesenchyme.
TS15: cranial preganglion	Present		1D CRABPII
TS15: branchial arch mesenchyme	Present		1D CRABPII
TS15: branchial pouch	Present	Regionally restricted	1D CRABPII	Present in pharyngeal pouch endoderm but absent from pharyngeal pouch ectoderm.
TS15: future brain	Present	Regionally restricted	1D CRABPII	Present in all brain vesicles; not homogeneously distributed; low levels in floor plate and ventral regions of neural epithelium of brain.
TS15: otocyst	Present		1D CRABPII
TS15: foregut	Present	Regionally restricted	1D CRABPII	Detected in foregut and midgut endoderm.
TS15: midgut	Present	Regionally restricted	1D CRABPII	Detected in foregut and midgut endoderm.
TS15: tail mesenchyme	Present		1D CRABPII

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present		2A CRABPII	Transcripts detected in neural epithelium at all levels of the craniocaudal axis.
TS12: future hindbrain	Present	Regionally restricted	2A CRABPII	Transcripts present in rhombomeres caudal to the preotic sulcus; highest expression in more caudal regions, extending further caudally.
TS12: posterior pro-rhombomere	Present		2A CRABPII
TS12: head mesenchyme	Present		2A CRABPII	Diffuse labelling in the cranial mesenchyme.
TS12: trunk unsegmented mesenchyme	Present	Regionally restricted	2A CRABPII	More intense signal in undifferentiated presomitic mesenchyme.

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present		2B CRABPII	Transcripts detected in neural epithelium at all levels of the craniocaudal axis, including forebrain.
TS12: neural ectoderm	Present		2B CRABPII	Transcripts detected in neural epithelium at all levels of the craniocaudal axis, including forebrain.
TS12: future hindbrain	Present	Regionally restricted	2B CRABPII	Highest expression in neuroepithelium immediately caudal to the otic placode (rhombomere C).
TS12: otic placode	Present		2B CRABPII

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		2C CRABPII	Transcripts detected in neural epithelium at all levels of the craniocaudal axis.
TS13: future hindbrain posterior to rhombomere 5	Present	Regionally restricted	2C CRABPII	Highest expression in neuroepithelium immediately caudal to the otic placode (rhombomere C).
TS13: head mesenchyme	Present		2C CRABPII	Diffuse labelling in the cranial mesenchyme.
TS13: trunk unsegmented mesenchyme	Present	Regionally restricted	2C CRABPII	More intense signal in undifferentiated presomitic mesenchyme.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 1	Present		2D CRABPII
TS13: rhombomere 2	Present		2D CRABPII
TS13: rhombomere 2 neural crest	Present		2D CRABPII
TS13: rhombomere 3	Present		2D CRABPII
TS13: rhombomere 4	Strong		2D CRABPII
TS13: rhombomere 4 neural crest	Strong		2D CRABPII
TS13: rhombomere 5	Weak		2D CRABPII
TS13: future hindbrain posterior to rhombomere 5	Strong		2D CRABPII
TS13: future hindbrain posterior to rhombomere 5 neural crest	Weak		2D CRABPII
TS13: future spinal cord	Strong		2D CRABPII

Structure	Level	Pattern	Image	Note
TS14: facio-acoustic neural crest	Present		2E CRABPII
TS14: rhombomere	Present		2E CRABPII
TS14: trigeminal neural crest	Present		2E CRABPII
TS14: head mesenchyme derived from neural crest	Present	Regionally restricted	2E CRABPII	Relatively weaker signal in neural crest cells that will contribute to cranial ganglia IX and X (derived from rhombomeres 6 and 7).
TS14: future hindbrain	Present		2E CRABPII
TS14: future midbrain	Present		2E CRABPII
TS14: otic pit	Present		2E CRABPII	Otic vesicle was labelled.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3A CRABPII	No gradient detected in anteroposterior axis. Clear dorsoventral difference in labelling intensity; lower levels ventrally than dorsally. Only weak labelling in the most distal mesenchyme (progress zone) cells.
TS17: neural tube	Present	Regionally restricted	3A CRABPII	In the spinal cord, slightly higher levels in presumptive motor columns when compared to the rest of the neural tube.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3B CRABPII	No gradient detected in anteroposterior axis. Clear dorsoventral difference in labelling intensity; lower levels ventrally than dorsally. Only weak labelling in the most distal mesenchyme (progress zone) cells.
TS17: neural tube	Present	Regionally restricted	3B CRABPII	Slightly higher levels in presumptive motor columns when compared to the rest of the neural tube.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3C CRABPII	No gradient detected in anteroposterior axis. Clear dorsoventral difference in labelling intensity; lower levels ventrally than dorsally. Only weak labelling in the most distal mesenchyme (progress zone) cells.
TS17: neural tube	Strong	Regionally restricted	3C CRABPII	Slightly higher levels in presumptive motor columns when compared to the rest of the neural tube.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3D CRABPII	No gradient detected in anteroposterior axis. Clear dorsoventral difference in labelling intensity; lower levels ventrally than dorsally. Only weak labelling in the most distal mesenchyme (progress zone) cells.
TS17: neural tube	Present	Regionally restricted	3D CRABPII	Slightly higher levels in presumptive motor columns when compared to the rest of the neural tube.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4A CRABPII	No gradient detected in anteroposterior axis. Only weak labelling in the most distal mesenchyme (progress zone) cells.

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Present	Regionally restricted	4B CRABPII	Transcripts in developing muscles and interdigital mesenchyme; absent from cartilage; present in distal mesenchyme underlying the AER at the tip of the digits.

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Absent		5A CRABPII
TS17: head mesenchyme	Present	Regionally restricted	5A CRABPII	Expression in frontonasal mesenchyme.
TS17: trunk dermomyotome	Strong	Regionally restricted	5A CRABPII	Transcripts particularly abundant in dermamyotome; myotomic portions of somites are more intensely labelled.
TS17: cranial ganglion	Present		5A CRABPII
TS17: forebrain	Present		5A CRABPII
TS17: nasal pit	Present		5A CRABPII
TS17: spinal ganglion	Present		5A CRABPII

Structure	Level	Pattern	Image	Note
TS17: trunk dermomyotome	Strong	Regionally restricted	5B CRABPII	Transcripts particularly abundant in dermamyotome; myotomic portions of somites are more intensely labelled.
TS17: trunk sclerotome	Present		5B CRABPII	Sclerotome cells were labelled but less intensely.
TS17: surface ectoderm	Absent		5B CRABPII
TS17: forelimb bud mesenchyme	Present	Regionally restricted	5B CRABPII	Transcripts present in mesenchyme of forelimb; differential intensity of signal between ventral and dorsal portions
TS17: hindlimb bud mesenchyme	Present	Uniform	5B CRABPII	Transcripts present in mesenchyme of hindlimbs; more homogeneous distribution of transcripts.
TS17: cranial ganglion	Present		5B CRABPII
TS17: spinal ganglion	Present		5B CRABPII
TS17: nasal pit	Present		5B CRABPII	Signal detected in olfactory placode.
TS17: mesonephric tubule	Present		5B CRABPII
TS17: mesonephros mesenchyme	Present		5B CRABPII

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present	Regionally restricted	5C CRABPII	Expression in pharyngeal arch mesenchyme.
TS17: forelimb bud mesenchyme	Present	Regionally restricted	5C CRABPII	Differential intensity of signal between ventral and dorsal portions of forelimb buds.
TS17: hindlimb bud mesenchyme	Present	Uniform	5C CRABPII
TS17: embryo mesenchyme	Present	Regionally restricted	5C CRABPII	Although expression is present in other mesenchymal populations, there is no expression in the mesenchyme of stroma close to dorsal mesentery.
TS17: stomach epithelium	Weak		5C CRABPII	Faint signal in epithelium of the developing stomach.

Structure	Level	Pattern	Image	Note
TS20: trunk mesenchyme	Present	Regionally restricted	6CRABPII	Sclerotome cells were labelled but less intensely.
TS20: brain	Present	Regionally restricted	6CRABPII	Transcripts in all brain vesicles, widely distributed; several regions showed a particularly intense signal.
TS20: forebrain	Present		6CRABPII
TS20: telencephalon	Present		6CRABPII
TS20: hindbrain	Present	Regionally restricted	6CRABPII	In metencephalon and myelencephalon, cells in mantle layer were more intensely labelled than those of neuroepithelial and marginal layers.
TS20: metencephalon	Present	Regionally restricted	6CRABPII	In metencephalon and myelencephalon, cells in mantle layer were more intensely labelled than those of neuroepithelial and marginal layers.
TS20: midbrain	Present	Uniform	6CRABPII
TS20: cranial ganglion	Present		6CRABPII
TS20: spinal cord	Present		6CRABPII
TS20: spinal ganglion	Present		6CRABPII
TS20: optic stalk	Present		6CRABPII	Authors report expression around the optic stalk.
TS20: fronto-nasal process	Strong	Regionally restricted	6CRABPII	Abundant in distal frontonasal mesenchyme.
TS20: mandibular process	Strong		6CRABPII
TS20: tongue	Strong		6CRABPII
TS20: liver	Present	Uniform	6CRABPII	Low levels; diffuse labelling.
TS20: genital tubercle	Present		6CRABPII
TS20: metanephros	Present		6CRABPII

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	7A CRABPII	Transcripts widely distributed in the brain, but several regions showed a particularly intense signal.
TS20: telencephalon	Present		7A CRABPII
TS20: fronto-nasal process	Present	Regionally restricted	7A CRABPII	Abundant in distal frontonasal mesenchyme.
TS20: thymus primordium	Present		7A CRABPII
TS20: mandibular process	Strong		7A CRABPII
TS20: tongue	Strong		7A CRABPII

Structure	Level	Pattern	Image	Note
TS21: esophagus	Present		7B CRABPII
TS21: thymus primordium	Present		7B CRABPII

Structure	Level	Pattern	Image	Note
TS17: trunk dermomyotome	Strong		7C CRABPII	Transcripts particularly abundant in dermamyotome.
TS17: mesonephric tubule	Present		7C CRABPII
TS17: mesonephros mesenchyme	Present		7C CRABPII

Structure	Level	Pattern	Image	Note
TS20: gut epithelium	Absent		7D CRABPII	No expression in gut epithelium.
TS20: mesonephros	Absent		7D CRABPII
TS20: metanephros	Strong		7D CRABPII
TS20: genital tubercle	Present	Regionally restricted	7D CRABPII	Labelling in the periphery of genital tubercle mesenchyme; also at low levels in the core mesenchyme

Structure	Level	Pattern	Image	Note
TS22: metanephros	Present	Regionally restricted	7E CRABPII	Present in both stromal cells and nephrogenic tubules of the kidney.

Structure	Level	Pattern	Image	Note
TS22: pons	Present		8A CRABPII
TS22: telencephalon	Present		8A CRABPII
TS22: thalamus	Present		8A CRABPII
TS22: nasal cavity respiratory epithelium	Strong		8A CRABPII
TS22: olfactory epithelium	Strong		8A CRABPII

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present		8B CRABPII
TS26: thalamus	Present		8B CRABPII
TS26: hindbrain meninges	Absent		8B CRABPII	Not expressed in meninges covering the brainstem.

Structure	Level	Pattern	Image	Note
TS26: vagus ganglion	Present	Regionally restricted	8C CRABPII	Present in dorsal efferent nucleus of the vagus nerve.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present		9A CRABPII
TS17: infundibular recess of 3rd ventricle	Present		9A CRABPII

Structure	Level	Pattern	Image	Note
TS20: brain	Present	Regionally restricted	9B CRABPII	Transcripts widely distributed in the brain, but several regions showed a particularly intense signal.
TS20: adenohypophysis	Present		9B CRABPII
TS20: neurohypophysis	Present		9B CRABPII
TS20: telencephalon	Present		9B CRABPII
TS20: fronto-nasal process	Strong	Regionally restricted	9B CRABPII	Present in distal frontonasal mesenchyme.
TS20: mandibular process	Strong		9B CRABPII
TS20: tongue	Strong		9B CRABPII

Structure	Level	Pattern	Image	Note
TS21: cranial ganglion	Present		9C CRABPII
TS21: spinal cord floor plate	Absent		9C CRABPII
TS21: spinal cord	Present	Regionally restricted	9C CRABPII	Transcripts preferentially detected in the motor columns in the basal plate and in newly formed neuroblasts in the alar plate. No transcripts in neuroepithelial cell layer or floor and roof plates.
TS21: alar columns	Present	Regionally restricted	9C CRABPII	Transcripts in newly formed neuroblasts in the alar plate.
TS21: basal columns	Present	Regionally restricted	9C CRABPII	Transcripts preferentially detected in the motor columns in the basal plate.
TS21: spinal cord roof plate	Absent		9C CRABPII
TS21: spinal ganglion	Present		9C CRABPII
TS21: axial skeleton	Absent		9C CRABPII	No signficant levels in somite-derived cartilage (vertebra).

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Present	Uniform	10A CRABPII

Structure	Level	Pattern	Image	Note
TS21: olfactory epithelium	Strong		10B CRABPII	Transcripts in both apical and basal layers of olfactory epithelium.
TS21: nasal cavity respiratory epithelium	Strong		10B CRABPII

Structure	Level	Pattern	Image	Note
TS21: dermis	Present		10C CRABPII
TS21: epidermis	Absent		10C CRABPII
TS21: vibrissa dermal component	Present	Regionally restricted	10C CRABPII	Labelling in the mesenchymal (dermal) component of the whisker follicles.
TS21: vertebral axis musculature	Present		10C CRABPII	Present in differentiating skeletal muscle.

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Weak			Weak staining was noted in migrating neural crest cells in the cranial neuroepithelium.
TS13: neural tube	Weak	Regionally restricted		Most caudal parts of neural tube and tail bud showed a signal slightly above the background.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Absent
TS17: forelimb bud ectoderm	Absent
TS17: lung	Absent			No labelling above background in the lung epithelium.

Structure	Level	Pattern	Image	Note
TS20: limb	Present	Regionally restricted		Transcripts widely distributed in presumptive non-chondrogenic mesenchyme of the limb; absent from precartilage condensations and differentiated cartilage.
TS20: diencephalon	Present	Regionally restricted		Labelling in mantle layer of diencephalon.
TS20: trunk mesenchyme	Present	Regionally restricted		Present in differentiating skeletal muscle.
TS20: adenohypophysis	Present
TS20: diencephalon lateral wall	Present
TS20: remnant of Rathke's pouch	Present
TS20: lung	Absent			No labelling above background in the lung epithelium.

Structure	Level	Pattern	Image	Note
TS21: neurohypophysis infundibulum	Present
TS21: remnant of Rathke's pouch	Present
TS21: corpus striatum	Absent
TS21: inner ear	Present	Regionally restricted		Transcripts in inner ear sensory epithelium.
TS21: stomach	Present	Regionally restricted		Transcripts in stomach epithelium.
TS21: genital tubercle	Present	Regionally restricted		Labelling of genital tubercle mesenchyme.
TS21: lung	Absent

Structure	Level	Pattern	Image	Note
TS22: neurohypophysis infundibulum	Present
TS22: remnant of Rathke's pouch	Present
TS22: facial nerve	Present	Regionally restricted		Transcripts in motor nuclei of trigeminal and facial nerve.
TS22: trigeminal nerve	Present	Regionally restricted		Transcripts in motor nuclei of trigeminal and facial nerve.
TS22: inner ear	Present	Regionally restricted		Transcripts in inner ear sensory epithelium.
TS22: genital tubercle	Present	Regionally restricted		Labelling of genital tubercle mesenchyme.
TS22: lung	Absent			No labelling above background in the lung epithelium.
TS22: limb	Present	Regionally restricted		Transcripts in connective tissues (tendons, ligaments and perichondrium) of the limb.