Probe:

Hic-5 probe

Structure	Level	Pattern	Image	Note
TS15: heart atrium	Present		7B
TS15: primitive ventricle	Present		7B
TS15: trunk somite	Present		7B

Structure	Level	Pattern	Image	Note
TS17: heart atrium	Present
TS17: primitive ventricle	Present
TS17: trunk somite	Present

Structure	Level	Pattern	Image	Note
TS13: heart	Weak		8A	Weak to moderate expression in the heart tube.

Structure	Level	Pattern	Image	Note
TS17: heart	Present		8B
TS17: trunk somite	Present	Regionally restricted	8B	Expression in the myotome layer.

Structure	Level	Pattern	Image	Note
TS20: gut	Strong			Expression in the smooth muscle layer of the intestinal tract.
TS20: heart	Present
TS20: intraembryonic coelom	Weak			Weak signal in the mesenchymal tissue around the bronchial buds of the developing lung and in the developing diaphragm.
TS20: left lung lobar bronchus mesenchyme	Weak			Weak signal in the mesenchymal tissue around the bronchial buds of the developing lung and in the developing diaphragm.
TS20: right lung lobar bronchus mesenchyme	Weak			Weak signal in the mesenchymal tissue around the bronchial buds of the developing lung and in the developing diaphragm.

Structure	Level	Pattern	Image	Note
TS22: left atrium	Present		8C
TS22: right atrium	Present		8C
TS22: left ventricle cardiac muscle	Weak		8C
TS22: right ventricle cardiac muscle	Weak		8C

Structure	Level	Pattern	Image	Note
TS22: stomach	Strong	Regionally restricted	8D	Expression in the developing smooth muscle layer.
TS22: liver	Present	Single cells	8D	There was expression in the megakaryocytes.

Structure	Level	Pattern	Image	Note
TS22: lobar bronchus mesenchyme	Strong	Regionally restricted	8E	Expression in the mesenchymal stromal layer of the bronchial airways.

Structure	Level	Pattern	Image	Note
TS22: gut	Strong			Expression in the muscle layers of the intestinal tract
TS22: mesenchyme of metanephros	Present
TS22: diaphragm	Strong
TS22: axial muscle	Strong			Expression in developing spinal muscles

Structure	Level	Pattern	Image	Note
TS24: bladder	Strong	Regionally restricted	8F	Expression in the wall of the intestine and bladder.
TS24: gut	Strong	Regionally restricted	8F	Expression in the wall of the intestine and bladder.

Structure	Level	Pattern	Image	Note
TS24: lobar bronchus	Present	Regionally restricted		Expression in the muscle layers surrounding the bronchial airways.
TS24: main bronchus	Present	Regionally restricted		Expression in the muscle layers surrounding the bronchial airways.
TS24: ureter	Present	Regionally restricted		Expression in the muscle layers surrounding the bronchial airways.
TS24: vertebral axis musculature	Present	Regionally restricted		Expression in the developing muscles in the heart and neck region

Structure	Level	Pattern	Image	Note
TS24: heart ventricle	Weak		8G
TS24: liver	Present	Single cells	8G	Expression in megakaryocytes.
TS24: cardiac muscle tissue	Weak		8G

Structure	Level	Pattern	Image	Note
TS24: intercostal muscle	Present		8H
TS24: rib	Absent		8H