Probe:	Six3 probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	5A/B	Expression in anterior head fold; strongest in the most rostral part.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	6A/B	Expression in diencephalic part of ventral forebrain.
TS15: optic recess	Present		6A/B

Structure	Level	Pattern	Image	Note
TS15: Rathke's pouch	Present

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Weak		9A/B
TS20: medial ganglionic eminence	Weak		9A/B
TS20: midbrain	Present	Regionally restricted	9A/B	Expression in the midbrain tegmentum and posterior part of the pretectum.
TS20: thalamus	Present	Regionally restricted	9A/B	Expression in the ventral thalamus.
TS20: hypothalamus	Present		9A/B

Structure	Level	Pattern	Image	Note
TS20: remnant of Rathke's pouch	Present

Structure	Level	Pattern	Image	Note
TS22: midbrain	Strong	Regionally restricted	10A/B	Expression in pretectum.
TS22: midbrain tegmentum	Strong		10A/B

Structure	Level	Pattern	Image	Note
TS22: midbrain tegmentum	Strong		10C/D

Structure	Level	Pattern	Image	Note
TS22: lateral ganglionic eminence	Present		10E/F
TS22: medial ganglionic eminence	Present		10E/F
TS22: hypothalamus	Present		10E/F

Structure	Level	Pattern	Image	Note
TS22: lateral ganglionic eminence	Present		10G/H
TS22: medial ganglionic eminence	Present		10G/H
TS22: telencephalon septum	Present		10G/H
TS22: hypothalamus	Present		10G/H

Structure	Level	Pattern	Image	Note
TS22: eye	Strong		10I/J
TS22: optic chiasma	Strong		10I/J
TS22: telencephalon	Strong	Regionally restricted	10I/J	Expression in septum.

Structure	Level	Pattern	Image	Note
TS22: eye	Present		10K/L
TS22: hypothalamus	Present		10K/L
TS22: pituitary gland	Present		10K/L
TS22: telencephalon	Present	Regionally restricted	10K/L	Expression in septum.

Structure	Level	Pattern	Image	Note
TS15: optic stalk	Present		11A/B
TS15: optic vesicle	Present		11A/B

Structure	Level	Pattern	Image	Note
TS17: optic cup	Strong	Regionally restricted	11C/D	Strong expression in neural retina; no specific expression in pigmented layer.
TS17: lens pit	Strong		11C/D
TS17: eye surface ectoderm	Absent		11C/D	No specific expression in the corneal ectoderm.

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Strong		11E/F
TS19: neural retinal epithelium	Strong		11E/F
TS19: optic stalk	Present		11E/F

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Strong	Regionally restricted	11G/H	Expression is stronger in the inner neuroblastic layer.
TS21: lens fiber	Weak		11G/H
TS21: lens anterior epithelium	Strong		11G/H
TS21: optic stalk	Weak	Regionally restricted	11G/H	Expression in the nerve fibers in the optic stalk.
TS21: pigmented retinal epithelium	Absent		11G/H

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present	Regionally restricted	11I/J	There is a clear boundary of expression with strong staining in the inner neuroblastic layer and weaker staining in the outer neuroblastic layer.
TS22: lens anterior epithelium	Strong		11I/J

Structure	Level	Pattern	Image	Note
TS26: retina inner nuclear layer	Weak		11K/L	Weak expression in eyes; only in inner neuroblastic layer of the retina.

Structure	Level	Pattern	Image	Note
TS26: nasal cavity	Weak

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	12A/B	Expression in the septum as in wild type.
TS20: hypothalamus	Present		12A/B	Expression is the same as wild type.
TS20: lateral ganglionic eminence	Present		12A/B	Expression is the same as wild type.
TS20: medial ganglionic eminence	Present		12A/B	Expression is the same as wild type.
TS20: neurohypophysis infundibulum	Present		12A/B	Expression is the same as wild type.

Structure	Level	Pattern	Image	Note
TS20: remnant of Rathke's pouch	Present		12C/D	Expression is the same as wild type.