Gene Expression Data
Assay Details
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Assay
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Reference:
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J:30342
Oliver G, et al., Six3, a murine homologue of the sine oculis gene, demarcates the most anterior border of the developing neural plate and is expressed during eye development. Development. 1995 Dec;121(12):4045-55
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2148953
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Gene symbol:
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Six3
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Gene name:
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sine oculis-related homeobox 3
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Probe:
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Six3 probe2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
not shown:
embryonic day 6.5
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 6.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo |
Weak |
Regionally restricted |
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First detected expression; faint signal at anterior border of embryo
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Specimen
3A:
embryonic day 8.2
(more )
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.2
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Present |
Regionally restricted |
3A
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Restricted to most anterior border of neural plate, represented by the neural ridge; no expression in remainder of embryo.
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| TS12: neural ectoderm |
Present |
Regionally restricted |
3A
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Restricted to most anterior border of neural plate, represented by the neural ridge; no expression in remainder of embryo.
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Specimen
3B:
embryonic day 8.5
(more )
Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future prosencephalon |
Present |
Regionally restricted |
3B
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Expressing region has expanded further; located over anterior neural ridge; now covers part of the area corresonding to the prospective forebrain; also over adjacent region of neural plate.
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| TS13: neural ectoderm |
Present |
Regionally restricted |
3B
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Expressing region has expanded further; located over anterior neural ridge; now covers part of the area corresonding to the prospective forebrain; also over adjacent region of neural plate.
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Specimen
3C:
embryonic day 8.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
7A, 7B:
embryonic day 9.5
(more )
Specimen
7A, 7B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
8A:
embryonic day 11.5
(more )
Specimen
8A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
8B:
embryonic day 12.5
(more )
Specimen
8B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools