Gene Expression Data
Assay Details
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Assay
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Reference:
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J:52601
Campione M, et al., The homeobox gene Pitx2: mediator of asymmetric left-right signaling in vertebrate heart and gut looping. Development. 1999 Mar;126(6):1225-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2148888
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Gene symbol:
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Pitx2
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Gene name:
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paired-like homeodomain transcription factor 2
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Results
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Specimen
2A:
embryonic day 7.0
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo mesoderm |
Present |
Regionally restricted |
2A
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Expression was present in two, bilateral patches in the head mesenchyme.
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Specimen
2B:
embryonic day 8.0
(more )
Note: Specimen has 6 somites.
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Specimen has 6 somites.
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Specimen
2C:
embryonic day 9.0
(more )
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2D:
embryonic day 9.0
(more )
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4D:
embryonic day 9.5
(more )
Specimen
4D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: heart |
Present |
Regionally restricted |
4D
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Expression is predominantly on the left side.
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| TS15: common atrial chamber |
Strong |
Regionally restricted |
4D
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Expression is present on the left side of the ventral midline.
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Specimen
4E:
embryonic day 9.5
(more )
Specimen
4E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: heart |
Present |
Regionally restricted |
4E
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Expression is predominantly on the left side.
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| TS15: common atrial chamber |
Strong |
Regionally restricted |
4E
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Expression is present on the left side of the ventral midline.
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| TS15: outflow tract |
Present |
Regionally restricted |
4E
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Expression is confined to the left side but is less pronounced than in the atrium.
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| TS15: primitive ventricle |
Present |
Regionally restricted |
4E
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Expression is confined to the left side but is less pronounced than in the atrium.
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Specimen
4F:
embryonic day 9.5
(more )
Specimen
4F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: heart |
Present |
Regionally restricted |
4F
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Expression is predominantly on the left side.
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| TS15: outflow tract |
Present |
Regionally restricted |
4F
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Expression is confined to the left side but is less pronounced than in the atrium.
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| TS15: primitive ventricle |
Present |
Regionally restricted |
4F
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Expression is confined to the left side but is less pronounced than in the atrium.
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Specimen
4G:
embryonic day 11.0
(more )
Note: Specimen has 44-48 somites.
Specimen
4G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Specimen has 44-48 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: stomach |
Present |
Regionally restricted |
4G
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Expression is present solely on the left side (in the greater curvature).
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| TS18: midgut |
Present |
Regionally restricted |
4G
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Expression is present at the apex of the turning midgut loop, at the junction of between the large and small intestine.
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Specimen
4H:
embryonic day 13.5
(more )
Specimen
4H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: stomach |
Present |
Regionally restricted |
4H
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Expression is present solely on the left side (in the greater curvature).
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Specimen
Not shown:
embryonic day 13.5
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindgut |
Present |
Regionally restricted |
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Expression is present in the caecum.
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Specimen
4I:
embryonic day 16.5
(more )
Specimen
4I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: stomach |
Present |
Regionally restricted |
4I
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Expression is present solely on the left side (in the greater curvature).
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| TS24: gut |
Present |
Regionally restricted |
4I
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Expression is present in the caecum. The distal half was uniformly stained. In the proximal part (up to the junction with the small intestine), the staining was restricted to the outer curvature.
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Specimen
5A:
embryonic day 8.5; Dnah11iv/ Dnah11iv
(more )
Note: Specimen has 8-12 somites.
Specimen
5A:
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Specimen has 8-12 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: head mesenchyme |
Present |
Regionally restricted |
5A
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Expression is present in two, bilateral patches.
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| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
5A
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Expression is present on the left side of the lateral plate of this specimen. In the lateral plate mesoderm of mutant animals, 28% had expression on the left side, 25% on the right side, 31% on both sides, and 16% had no expression.
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Specimen
5B:
embryonic day 8.5; Dnah11iv/ Dnah11iv
(more )
Note: Specimen has 8-12 somites.
Specimen
5B:
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Specimen has 8-12 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: head mesenchyme |
Present |
Regionally restricted |
5B
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Expression is present in two, bilateral patches.
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| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
5B
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Expression is present on the right side of the lateral plate of this specimen. In the lateral plate mesoderm of mutant animals, 28% had expression on the left side, 25% on the right side, 31% on both sides, and 16% had no expression.
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Specimen
5C:
embryonic day 8.5; Dnah11iv/ Dnah11iv
(more )
Note: Specimen has 8-12 somites.
Specimen
5C:
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Specimen has 8-12 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: head mesenchyme |
Present |
Regionally restricted |
5C
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Expression is present in two, bilateral patches.
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| TS13: lateral plate mesoderm |
Present |
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5C
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Expression is present on both sides of the lateral plate of this specimen. In the lateral plate mesoderm of mutant animals, 28% had expression on the left side, 25% on the right side, 31% on both sides, and 16% had no expression.
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Specimen
5D:
embryonic day 8.5; Dnah11iv/ Dnah11iv
(more )
Note: Specimen has 8-12 somites.
Specimen
5D:
(close )
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Genetic Background:
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involves: C3H
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Mutant Allele(s):
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Dnah11iv/Dnah11iv
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Specimen has 8-12 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: head mesenchyme |
Present |
Regionally restricted |
5D
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Expression is present in two, bilateral patches.
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| TS13: lateral plate mesoderm |
Absent |
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5D
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There was no expression in the lateral plate of this specimen. In the lateral plate mesoderm of mutant animals, 28% had expression on the left side, 25% on the right side, 31% on both sides, and 16% had no expression.
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