|
Results
|
Specimen
5I,5J:
embryonic day 8.0
(more  )
Note: 3-somite stage.
Specimen
5I,5J:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
3-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future midbrain |
Present |
Regionally restricted |
5I
,
5J
|
Authors report Pax2 expression in the caudal portion of the Otx2-positive region (see assay MGI:2148282); overlaps posterior limit of Emx2 expression (see assay MGI:2148290); extends beyond caudal limit of Otx2 expression.
|
Specimen
5Q,5R:
embryonic day 8.0; Emx2tm1Sia/ Emx2tm1Sia, Otx2tm1Sia/Otx2 +
(more )
Note: 3-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Specimen
5Q,5R:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Emx2tm1Sia/Emx2tm1Sia
Otx2tm1Sia/Otx2+
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
3-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future midbrain |
Present |
Regionally restricted |
5Q
,
5R
|
Expression is more diffuse and expanded rostrally.
|
Specimen
5K,5L,5U:
embryonic day 8.0
(more )
Note: 4-somite stage.
Specimen
5K,5L,5U:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
4-somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future prosencephalon |
Present |
|
5K
,
5L
|
|
| TS12: future hindbrain |
Present |
Regionally restricted |
5L
,
5K
|
Expression extends caudally into preotic sulcus at the most lateral (future dorsal) edge; in medial (future lateral and ventral) region, expression terminates more rostrally; corresponds to future r1 and/or r2.
|
| TS12: pre-otic sulcus |
Present |
Regionally restricted |
5L
,
5K
|
Expression extends caudally into preotic sulcus at the most lateral (future dorsal) edge; in medial (future lateral and ventral) region, expression terminates more rostrally; corresponds to future r1 and/or r2.
|
| TS12: future midbrain |
Present |
Regionally restricted |
5U
|
Anterior Pax2-negative domain.
|
Specimen
5S,5T,5V:
embryonic day 8.0; Emx2tm1Sia/ Emx2tm1Sia, Otx2tm1Sia/Otx2 +
(more )
Note: 4-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Specimen
5S,5T,5V:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Emx2tm1Sia/Emx2tm1Sia
Otx2tm1Sia/Otx2+
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
4-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future midbrain |
Present |
Regionally restricted |
5S
,
5V
,
5T
|
Caudal limit of expression in the medial region shifted rostrally; lateral positive and medial negative regions expanded; anterior Pax2-negative domain greatly reduced.
|
|
Analysis Tools