Six3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:70745 Suda Y, et al., Emx2 directs the development of diencephalon in cooperation with Otx2. Development. 2001 Jul;128(13):2433-50
Assay type:	RNA in situ
MGI Accession ID:	MGI:2148289
Gene symbol:	Six3
Gene name:	sine oculis-related homeobox 3

Probe:	Six3 probe2
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

4M ©

Specimen 4M: embryonic day 8.0 (more

)
Note: 6-somite stage.

Structure	Level	Pattern	Image	Note
TS12: future brain	Present	Regionally restricted	4M	Expression in the most rostral neuroectoderm.

4N ©

Specimen 4N: embryonic day 8.0; Emx2^tm1Sia/Emx2^tm1Sia, Otx2^tm1Sia/Otx2⁺ (more

)
Note: 6-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.

Structure	Level	Pattern	Image	Note
TS12: future brain	Present	Regionally restricted	4N	Authors report normal expression in double mutants.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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