En1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:70745 Suda Y, et al., Emx2 directs the development of diencephalon in cooperation with Otx2. Development. 2001 Jul;128(13):2433-50
Assay type:	RNA in situ
MGI Accession ID:	MGI:2148288
Gene symbol:	En1
Gene name:	engrailed 1

Probe:	En1 probe4
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

4G ©

Specimen 4G: embryonic day 8.0 (more

)
Note: 6-somite stage.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present	Regionally restricted	4G	Authors report complete regression to the caudal portion of the prospective mesencephalon.

4H ©

Specimen 4H: embryonic day 8.0; Emx2^tm1Sia/Emx2^tm1Sia, Otx2^tm1Sia/Otx2⁺ (more

)
Note: 6-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present	Regionally restricted	4H	Expansion of positive domain accompanied by an anterior shift.

8G ©

Specimen 8G: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present		8G

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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