Gene Expression Data
Assay Details
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Assay
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Reference:
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J:70745
Suda Y, et al., Emx2 directs the development of diencephalon in cooperation with Otx2. Development. 2001 Jul;128(13):2433-50
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2148287
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Gene symbol:
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Fgf8
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Gene name:
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fibroblast growth factor 8
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Probe:
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Fgf8 cDNA13
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3M:
embryonic day 9.5
(more  )
Specimen
3M:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: telencephalon |
Present |
Regionally restricted |
3M
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Expression in anterior neural ridge and roof at position of telodiencephalicus.
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| TS15: diencephalon |
Present |
Regionally restricted |
3M
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Expression in anterior neural ridge and roof at position of telodiencephalicus.
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| TS15: future hindbrain |
Present |
Regionally restricted |
3M
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Expression at isthmus.
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| TS15: future midbrain |
Present |
Regionally restricted |
3M
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Expression at isthmus.
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Specimen
3N:
embryonic day 9.5; Emx2tm1Sia/ Emx2tm1Sia, Otx2tm1Sia/Otx2 +
(more )
Note: Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Specimen
3N:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: telencephalon |
Present |
Regionally restricted |
3N
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Expression in the anterior neural ridge; absent in the roof at the position of the telodiencephalicus or the roof of the ventral thalamus.
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| TS15: diencephalon |
Present |
Regionally restricted |
3N
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Expression in the anterior neural ridge; absent in the roof at the position of the telodiencephalicus or the roof of the ventral thalamus.
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| TS15: future hindbrain |
Present |
Regionally restricted |
3N
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Region of isthmic expression shifted anteriorly in the double mutant.
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| TS15: future midbrain |
Present |
Regionally restricted |
3N
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Region of isthmic expression shifted anteriorly in the double mutant.
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Specimen
4K:
embryonic day 8.0
(more )
Note: 6-somite stage.
Specimen
4K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6-somite stage.
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Specimen
4L:
embryonic day 8.0; Emx2tm1Sia/ Emx2tm1Sia, Otx2tm1Sia/Otx2 +
(more )
Note: 6-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Specimen
4L:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future hindbrain |
Present |
Regionally restricted |
4L
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Expression at future isthmus, less distinct in double mutants.
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| TS12: future midbrain |
Present |
Regionally restricted |
4L
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Expression at future isthmus, less distinct in double mutants.
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Specimen
8K:
embryonic day 9.5
(more )
Specimen
8K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future forebrain |
Present |
Regionally restricted |
8K
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Expression was detected in the anterior neural ridge and the roof at the position of the telodiencephalicus.
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Analysis Tools