Gene Expression Data
Assay Details
Assay
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Reference:
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J:70745
Suda Y, et al., Emx2 directs the development of diencephalon in cooperation with Otx2. Development. 2001 Jul;128(13):2433-50
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2148286
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Gene symbol:
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Pax5
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Gene name:
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paired box 5
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Probe:
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5sg8
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3K:
embryonic day 9.5
(more )
Specimen
3K:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3L:
embryonic day 9.5; Emx2tm1Sia/ Emx2tm1Sia, Otx2tm1Sia/Otx2 +
(more )
Note: Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Specimen
3L:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia Otx2tm1Sia/Otx2+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
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Structure |
Level |
Pattern |
Image |
Note |
TS15: future hindbrain |
Present |
Regionally restricted |
3L
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Enlarged regions of expression both rostral and caudal to isthmic constriction; greatly reduced negative region extending up to the sulcus telodiencephalicus.
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TS15: future midbrain |
Present |
Regionally restricted |
3L
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Enlarged regions of expression both rostral and caudal to isthmic constriction; greatly reduced negative region extending up to the sulcus telodiencephalicus.
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Specimen
4I:
embryonic day 8.0
(more )
Note: 6-somite stage.
Specimen
4I:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6-somite stage.
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Structure |
Level |
Pattern |
Image |
Note |
TS12: future midbrain |
Present |
Regionally restricted |
4I
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Authors report complete regression to the caudal portion of the prospective mesencephalon.
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Specimen
4J:
embryonic day 8.0; Emx2tm1Sia/ Emx2tm1Sia, Otx2tm1Sia/Otx2 +
(more )
Note: 6-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Specimen
4J:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Note:
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6-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: future midbrain |
Present |
Regionally restricted |
4J
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Expansion of positive domain accompanied by an anterior shift.
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Specimen
8I:
embryonic day 10.5
(more )
Specimen
8I:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
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