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Results
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Specimen
3C:
embryonic day 9.5
(more  )
Specimen
3C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future forebrain |
Present |
Regionally restricted |
3C
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Expression in forebrain and midbrain with caudal limit at mid/hindbrain junction.
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| TS15: future midbrain |
Present |
Regionally restricted |
3C
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Expression in forebrain and midbrain with caudal limit at mid/hindbrain junction.
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Specimen
3D:
embryonic day 9.5; Emx2tm1Sia/ Emx2tm1Sia, Otx2tm1Sia/Otx2 +
(more )
Note: Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Specimen
3D:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 and Otx2 heterozygotes (from F3 and F5 generations, respectively) were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future forebrain |
Present |
Regionally restricted |
3D
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Expression was greatly reduced in double mutants, particularly in the region caudal to the sulcus telodiencephalicus. Caudal limit shifted rostrally and expression was restricted to the more dorsal region.
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| TS15: future midbrain |
Present |
Regionally restricted |
3D
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Expression was greatly reduced in double mutants, particularly in the region caudal to the sulcus telodiencephalicus. Caudal limit shifted rostrally and expression was restricted to the more dorsal region.
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Specimen
4A:
embryonic day 8.0; Otx2tm1Sia/Otx2 +
(more )
Note: 6-somite stage. Otx2 heterozygotes were maintained by crosses with CBA mice.
Specimen
4A:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6-somite stage. Otx2 heterozygotes were maintained by crosses with CBA mice.
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Specimen
4B:
embryonic day 8.0; Emx2tm1Sia/ Emx2tm1Sia, Otx2tm1Sia/Otx2 +
(more )
Note: 6-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Specimen
4B:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
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Specimen
5A,5B:
embryonic day 8.0
(more )
Note: 3-somite stage.
Specimen
5A,5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
5B
,
5A
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Authors report expression in anterior neuroectoderm.
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Specimen
5M,5N:
embryonic day 8.0; Emx2tm1Sia/ Emx2tm1Sia, Otx2tm1Sia/Otx2 +
(more )
Note: 3-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Specimen
5M,5N:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
5N
,
5M
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Otx2-positive domain was reduced both rostrocaudally and lateromedially (future dorsoventrally).
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Specimen
5C,5D:
embryonic day 8.0
(more )
Note: 4-somite stage.
Specimen
5C,5D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
5C
,
5D
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Authors report expression in anterior neuroectoderm.
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Specimen
5O,5P:
embryonic day 8.0; Emx2tm1Sia/ Emx2tm1Sia, Otx2tm1Sia/Otx2 +
(more )
Note: 4-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
Specimen
5O,5P:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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|
Note:
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4-somite stage. Emx2 and Otx2 heterozygotes were maintained by crosses with CBA mice. Single Emx2 (F3 gen.) and Otx2 (F5 gen.) heterozygotes were mated to generate double heterozygotes, which were subsequently mated to generate the double mutants.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future brain |
Present |
Regionally restricted |
5O
,
5P
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Rostrocaudal reduction of the Otx2-positive domain was more apparent at the 4-somite stage compared to the 6-somite stage.
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Analysis Tools