Probe:	pD7c8.1
Probe preparation:	Antisense labelled with P33 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	3B	Present at the anterior midline in the axial mesoderm. No expression was seen in other embryonic tissues.
TS11: embryo ectoderm	Absent		3B	Absent from the ectoderm overlying the anterior midline mesoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	3C	Present at the anterior midline in the axial mesoderm. No expression was seen in other embryonic tissues.
TS11: embryo ectoderm	Absent		3C	Absent from the anterior midline ectoderm.

Structure	Level	Pattern	Image	Note
TS11: extraembryonic component	Absent

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	3D	Present at the anterior midline in the axial mesoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	3E	Present at the anterior midline in the axial mesoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	3F	Present at the anterior midline in the axial mesoderm.

Structure	Level	Pattern	Image	Note
TS13: embryo mesenchyme	Present	Regionally restricted		Present at the anterior midline in the axial mesoderm.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Strong	Regionally restricted	4A/B	The expression encompasses 40 percent of the ventral neural tube at its maximum extent in the midbrain. Also present in a ring of cells in the ventral surface of the midbrain-diencephalic region.
TS15: diencephalon	Present	Regionally restricted	4A/B	Present in a small patch in the ventral diencephalon at the midline.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong	Regionally restricted	4C	Present at the ventral midline of the neural tube caudal to the hindbrain.
TS15: diencephalon	Present	Regionally restricted	4C	Present in the ventral diencephalon and in a ring of cells in the ventral surface of the midbrain-diencephalic region.
TS15: future forebrain	Strong	Regionally restricted	4C	Present in the ventral boundary region of the midbrain/forebrain.
TS15: telencephalon	Present		4C	The author states that expression is present rostral to the midbrain.T

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong	Regionally restricted	4D	Present at the ventral midline of the neural tube caudal to the hindbrain. This includes cells adjacent to the optic vesicle.
TS15: diencephalon	Present		4D	The authors state that expression is present rostral to the midbrain but that it splits and extends bilaterally.
TS15: telencephalon	Present		4D	The authors state that expression is present rostral to the midbrain but that it splits and extends bilaterally.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong	Regionally restricted	4E	Present at the ventral midline of the neural tube caudal to the hindbrain. This includes ventral neural tube cells overlying the pharngeal lumen and in cells adjacent to the optic vesicle.
TS15: diencephalon	Present	Regionally restricted	4E	Present in the ventral diencephalon and in a ring of cells in the ventral surface of the midbrain-diencephalic region. The author states that expression rostral to the midbrain splits and extends bilaterally, reuniting in the ventral midline.
TS15: telencephalon	Present	Regionally restricted	4E	The authors state that expression is present rostral to the midbrain but that it splits and extends bilaterally, reuniting in the ventral midline at the diencephalon.

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Strong	Uniform	4F

Structure	Level	Pattern	Image	Note
TS15: notochord	Strong	Uniform	4G , 4H
TS15: neural tube floor plate	Strong	Regionally restricted	4G , 4H	Present in the floorplate in pericardiac regions.

Structure	Level	Pattern	Image	Note
TS15: lung	Present		4I

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	5A/B	Present in most of the ventral neural tube.
TS17: diencephalon	Strong	Regionally restricted	5A/B	Present in the ventral region.
TS17: notochord	Strong	Uniform	5A/B
TS17: carina trachea	Strong		5A/B

Structure	Level	Pattern	Image	Note
TS17: midbrain lateral wall	Present	Regionally restricted	5C	Present in the ventral epithelium.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present	Uniform	5D
TS17: notochord	Strong	Uniform	5D

Structure	Level	Pattern	Image	Note
TS17: trachea	Present	Regionally restricted	5E	Present in the epithelia of the tracheal lumen.

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present	Uniform	5F
TS17: notochord	Strong	Uniform	5F
TS17: hindgut epithelium	Present		5F

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	5H	Expression is present in the mesoderm along the posterior margin of the limb buds. It is absent in the anterior mesoderm of the limb buds.
TS17: forelimb bud apical ectodermal ridge	Absent		5H
TS17: forelimb bud ectoderm	Absent		5H

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	5I	Expression is present in the mesoderm along the posterior margin of the limb buds. It is absent in the anterior mesoderm of the limb buds.
TS17: forelimb bud apical ectodermal ridge	Absent		5I
TS17: forelimb bud ectoderm	Absent		5I

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	5J	Expression is present in the mesoderm along the posterior margin of the limb buds. It is absent in the anterior mesoderm of the limb buds.
TS17: forelimb bud apical ectodermal ridge	Absent		5J
TS17: forelimb bud ectoderm	Absent		5J

Structure	Level	Pattern	Image	Note
TS17: foregut	Present	Regionally restricted		Present in the endoderm lining the foregut.
TS17: pharynx	Present	Regionally restricted		Present in the endoderm lining the future pharynx.
TS17: lung	Strong			Expression is more intense than in the pharynx and foregut.
TS17: hindgut	Present
TS17: hindlimb bud mesenchyme	Present	Regionally restricted		Expression is present in the mesoderm along the posterior margin of the limb buds. It is absent in the anterior mesoderm of the limb buds.
TS17: hindlimb bud apical ectodermal ridge	Absent
TS17: hindlimb bud ectoderm	Absent