Gene Expression Data
Assay Details
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Assay
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Reference:
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J:48569
Grove EA, et al., The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice. Development. 1998 Jun;125(12):2315-25
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2136866
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Gene symbol:
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Gli3
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Gene name:
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GLI-Kruppel family member GLI3
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Probe:
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pGli-3a
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Probe preparation:
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Antisense RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Authors report that the Gli3 probe was derived from a 0.8 kb mouse Gli3 cDNA (Hui et al., 1994). One- and two-color in situ hybridization was performed using digoxygenin- and/or fluorescein-labeled probes, detected with alkaline phosphatase-conjugated anti-digoxigenin or anti-fluorescein antibodies. Color reactions were carried out using different alkaline phosphatase substrates, resulting in different colored products as indicated in some of the figure legends.
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Results
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Specimen
not shown:
embryonic day 9.5
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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either: C3HeB/FeJ or CD-1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: telencephalon |
Present |
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Expression readily detected throughout telencephalic neuroepithelium.
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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C3HeB/FeJ
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6A:
embryonic day 12.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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either: C3HeB/FeJ or CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
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6A
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Expression readily detected throughout telencephalic neuroepithelium.
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Specimen
6B:
embryonic day 12.5
(more )
Note: This is a coronal section through the same brain (hybridized as a whole mount) as in Fig. 6A.
Specimen
6B:
(close )
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Genetic Background:
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either: C3HeB/FeJ or CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This is a coronal section through the same brain (hybridized as a whole mount) as in Fig. 6A.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
not shown:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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