Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:48569
Grove EA, et al., The hem of the embryonic cerebral cortex is defined by the expression of multiple Wnt genes and is compromised in Gli3-deficient mice. Development. 1998 Jun;125(12):2315-25
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:2136858
|
|
Gene symbol:
|
Wnt7a
|
|
Gene name:
|
wingless-type MMTV integration site family, member 7A
|
|
Probe:
|
Wnt7a probe2
|
|
Probe preparation:
|
Antisense RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
One- and two-color in situ hybridization was performed using digoxygenin- and/or fluorescein-labeled probes, detected with alkaline phosphatase-conjugated anti-digoxigenin or anti-fluorescein antibodies. Color reactions were carried out using different alkaline phosphatase substrates, resulting in different colored products as indicated in some of the figure legends.
|
|
|
Results
|
Specimen
1C:
embryonic day 12.5
(more  )
Specimen
1C:
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Strong |
Regionally restricted |
1C
|
Strong expression in the lateral and dorsal cerebral cortex.
|
Specimen
1G:
embryonic day 12.5
(more )
Specimen
1G:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Strong |
Regionally restricted |
1G
|
No expression at the medial margin of the cerebral hemisphere, the cortical hem.
|
Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
7C:
(close )
|
Genetic Background:
|
involves: C3HeB/FeJ
|
|
Mutant Allele(s):
|
Gli3Xt-J/Gli3Xt-J
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon |
Present |
Regionally restricted |
7C
|
In brains of non-exencephalic homozygous mutant mice, expression is maintained in the lateral and dorsal telencephalon.
|
|
Analysis Tools