Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:40605
Miyamoto N, et al., Defects of urogenital development in mice lacking Emx2. Development. 1997 May;124(9):1653-64
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:2136468
|
|
Gene symbol:
|
Wt1
|
|
Gene name:
|
WT1 transcription factor
|
|
Probe:
|
WT-1 probe1
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
6A:
embryonic day 11.5
(more  )
Specimen
6A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: metanephric mesenchyme |
Present |
|
6A
|
Expression throughout the metanephric mesenchyme with invading ureteric bud.
|
Specimen
6B:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Emx2tm1Sia/Emx2tm1Sia
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: metanephric mesenchyme |
Present |
|
6B
|
Authors state that expression in the metenephric mesenchyme is unaffected in the mutant.
|
Specimen
4J:
embryonic day 13.5
(more )
Specimen
4J:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: metanephros |
Present |
Regionally restricted |
4J
|
Expression in the condensed mesenchyme surrounding the bud of each ureteric branch, but not in the Wnt4-positive epithelializing mesenchyme.
|
|
Analysis Tools