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Gene Expression Data
Assay Details
Assay
Reference: J:1380 MacKenzie A, et al., Expression patterns of the homeobox gene, Hox-8, in the mouse embryo suggest a role in specifying tooth initiation and shape. Development. 1992 Jun;115(2):403-20
Assay type: RNA in situ
MGI Accession ID: MGI:2136261
Gene symbol: Msx1
Gene name: msh homeobox 1
Probe: Hox-7 cDNA
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Assay notes: E17.0 and E18.0 embryo heads were demineralized in 0.5M EDTA for 4 days, dehydrated through grades of ethanol, and cleared in chloroform prior to embedding in wax.
Results
Specimen 1 C: embryonic day 10.5-11.0 (more )
Structure Level Pattern Image Note
TS18: 1st branchial arch mandibular component mesenchyme derived from neural crest Present Regionally restricted 1C The expression domain is more restricted to the distal mesenchyme (ie aboral side) of each arch.
TS18: 1st branchial arch maxillary component mesenchyme derived from neural crest Present Regionally restricted 1C The expression domain is more restricted to the distal mesenchyme (ie aboral side) of each arch.
TS18: 2nd branchial arch mesenchyme derived from neural crest Present Regionally restricted 1C The expression domain is more restricted to the distal mesenchyme (ie aboral side) of each arch.
TS18: 3rd branchial arch mesenchyme derived from neural crest Present Regionally restricted 1C The expression domain is more restricted to the distal mesenchyme (ie aboral side) of each arch.
TS18: 4th branchial arch mesenchyme derived from neural crest Present Regionally restricted 1C The expression domain is more restricted to the distal mesenchyme (ie aboral side) of each arch.

Specimen 2 C: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: 1st branchial arch mandibular component mesenchyme Present 2C
TS18: latero-nasal process mesenchyme Present 2C
TS18: medial-nasal process mesenchyme Present 2C
TS18: 1st branchial arch maxillary component mesenchyme Present 2C

Specimen 5 C: embryonic day 12.0 (more )
Note: Bud stage of tooth development.
Structure Level Pattern Image Note
TS20: mandibular process mesenchyme Present Regionally restricted 5C Expression is confined to the mesenchyme of the dental papilla and dental follicle.
TS20: maxillary process mesenchyme Present Regionally restricted 5C Expression is confined to the mesenchyme of the dental papilla and dental follicle.

Specimen 6 C: embryonic day 15.0 (more )
Note: Cap stage of tooth development.
Structure Level Pattern Image Note
TS23: lower jaw molar dental follicle Present 6C Expression is restricted to the mesenchyme of the dental papilla and dental follicle.
TS23: lower jaw molar dental papilla Present 6C Expression is restricted to the mesenchyme of the dental papilla and dental follicle.
TS23: lower jaw molar enamel organ Absent 6C

Specimen 7 C: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: lower jaw molar dental follicle Present 7C Expression is restricted to the mesenchyme of the dental papilla and dental follicle.
TS24: lower jaw molar dental papilla Present 7C Expression is restricted to the mesenchyme of the dental papilla and dental follicle.
TS24: lower jaw molar enamel organ Absent 7C

Specimen 7 F: embryonic day 17.0 (more )
Structure Level Pattern Image Note
TS25: lower jaw molar dental follicle Present 7F Expression is restricted to the mesenchyme of the dental papilla and dental follicle.
TS25: lower jaw molar dental papilla Present 7F Expression is restricted to the mesenchyme of the dental papilla and dental follicle.

Specimen 8 C: embryonic day 18.0 (more )
Structure Level Pattern Image Note
TS26: lower jaw incisor dental papilla Present 8C Strong expression in the odontoblasts and subodontoblastic cells at the tips of the cusps, as well as in the dental follicle.
TS26: lower jaw incisor dental follicle Present 8C Strong expression in the odontoblasts and subodontoblastic cells at the tips of the cusps, as well as in the dental follicle.

Specimen 10 C: embryonic day 12.0 (more )
Structure Level Pattern Image Note
TS20: maxillary process epithelium Absent 10C
TS20: oral epithelium Absent 10C
TS20: maxilla Present Regionally restricted 10C Expression in the mesenchyme surrounding the invaginating dental epithelium, the distal mesenchyme of the lip, and the forming pre-maxillary bones.
TS20: maxillary process mesenchyme Present Regionally restricted 10C Expression in the mesenchyme surrounding the invaginating dental epithelium, the distal mesenchyme of the lip, and the forming pre-maxillary bones.

Specimen 11 C: embryonic day 13.5 (more )
Note: Bud stage of tooth development.
Structure Level Pattern Image Note
TS21: lower jaw incisor mesenchyme Present Regionally restricted 11C Expression in the incisor dental papilla and follicle.

Specimen 12 B: embryonic day 16.0 (more )
Note: Cap stage of tooth development.
Structure Level Pattern Image Note
TS24: lower jaw incisor dental follicle Present 12B Expression in the mesenchyme of the dental papilla and follicle.
TS24: lower jaw incisor dental papilla Present 12B Expression in the mesenchyme of the dental papilla and follicle.
TS24: upper jaw incisor dental papilla Present 12B Expression in the mesenchyme of the dental papilla and follicle.
TS24: dental sac Present 12B Expression in the mesenchyme of the upper jaw incisor dental follicle.
TS24: lower jaw incisor enamel organ Absent 12B
TS24: upper jaw incisor enamel organ Absent 12B
TS24: hair follicle Present 12B
TS24: vibrissa Present 12B

Specimen 13 C: embryonic day 16.0 (more )
Structure Level Pattern Image Note
TS24: lower jaw incisor dental papilla Present 13C
TS24: inner enamel epithelium Absent 13C Absent in the external enamel epithelia, stellate reticulum, and internal enamel epithelia.
TS24: lower jaw incisor enamel organ Absent 13C Absent in the external enamel epithelia, stellate reticulum, and internal enamel epithelia.
TS24: outer enamel epithelium Absent 13C Absent in the external enamel epithelia, stellate reticulum, and internal enamel epithelia.
TS24: stellate reticulum Absent 13C Absent in the external enamel epithelia, stellate reticulum, and internal enamel epithelia.

Specimen 14 C: embryonic day 18.0 (more )
Note: Bell stage of tooth development.
Structure Level Pattern Image Note
TS26: lower jaw incisor dental follicle Present Regionally restricted 14C Expression in the mesenchyme of the dental papilla (including the odontoblasts) and follicle.
TS26: lower jaw incisor dental papilla Present Regionally restricted 14C Expression in the mesenchyme of the dental papilla (including the odontoblasts) and follicle.

Specimen 15 C: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: cranium Present 15C
TS21: diencephalon meninges Present 15C Expression in the cellular precursors of the meninges.
TS21: hindbrain meninges Present 15C Expression in the cellular precursors of the meninges.
TS21: midbrain meninges Present 15C Expression in the cellular precursors of the meninges.
TS21: telencephalon meninges Present 15C Expression in the cellular precursors of the meninges.
TS21: lateral ventricle choroid plexus Present Regionally restricted 15C Expression in the neuroepithelium of the lateral choroid plexus.

Specimen 16 C: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: vomeronasal organ mesenchyme Present Regionally restricted 16C Expression is restricted to the mesenchymal condensation on the nasal side, with only slight labeling of the mesenchyme adjacent to the thickened abnasal side.

Specimen 16 F: embryonic day 15.0 (more )
Structure Level Pattern Image Note
TS23: vomeronasal organ mesenchyme Present Regionally restricted 16F Expression is restricted to the mesenchymal condensation on the nasal side, with only slight labeling of the mesenchyme adjacent to the thickened abnasal side.

Specimen 16 I: embryonic day 17.0 (more )
Structure Level Pattern Image Note
TS25: vomeronasal organ mesenchyme Present Regionally restricted 16I Expression is restricted to the mesenchymal condensation on the nasal side, with only slight labeling of the mesenchyme adjacent to the thickened abnasal side.

Specimen not shown: embryonic day 12.0 (more )
Structure Level Pattern Image Note
TS20: adenohypophysis Present
TS20: endolymphatic appendage Absent


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory