Probe:	Tgfb3 probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent

Structure	Level	Pattern	Image	Note
TS17: axial skeleton	Present	Regionally restricted		The earliest abundant expression was seen at 10.5 dpc and was restricted to intervertebral disc anlagen.

Structure	Level	Pattern	Image	Note
TS20: limb	Absent		3L

Structure	Level	Pattern	Image	Note
TS20: axial skeleton	Present	Regionally restricted	4A/B	Expression in intervertebral disc anlagen.
TS20: lung	Present	Regionally restricted	4A/B	Submucosal expression in trachea and proximal bronchi; hybridization to columnar epithelium and subepithelially in the bronchus; intense expression in immature columnar epithelial cells of the growing bronchioles; also present in pulmonary mesothelia.

Structure	Level	Pattern	Image	Note
TS20: vibrissa	Present			Expression in invaginating whisker follicles.

Structure	Level	Pattern	Image	Note
TS22: heart	Present	Regionally restricted	2I	Cardiac expression is limited to mesenchymal condensations at the base of the heart valves.

Structure	Level	Pattern	Image	Note
TS22: axial skeleton tail region	Present	Regionally restricted	3C	Expression restricted to intervertebral disc anlagen.

Structure	Level	Pattern	Image	Note
TS22: trachea mesenchyme	Present	Regionally restricted	3G	Abundant expression in mesenchyme within and adjacent to perichondria of non-ossifying cartilage, including tracheal cartilage.

Structure	Level	Pattern	Image	Note
TS22: axial skeleton	Present	Regionally restricted	3I/J	Transcripts observed in perichondria of cartilage models of the vertebrae and ribs; associated with areas of precartilage condensation and active growth and differentiation of chondroblasts; not expressed in hypertrophic chondrocytes.
TS22: rib cartilage condensation	Present	Regionally restricted	3I/J	Transcripts observed in perichondria of cartilage models of the vertebrae and ribs; associated with areas of precartilage condensation and active growth and differentiation of chondroblasts; not expressed in hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS22: limb	Absent			No transcripts observed at any stage in chondrification or ossification of the major long bones.

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Regionally restricted	4F	Expression restricted to columnar epithelial cells of the proximal respiratory tree; no expression in distal cuboidal epithelium; no expression in the epithelia of the growing terminal end buds; no expression in differentiated epithelium of the alveoli

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Regionally restricted	4H	Expression restricted to columnar epithelial cells of the proximal respiratory tree; no expression in distal cuboidal epithelium; no expression in the epithelia of the growing terminal end buds; no expression in differentiated epithelium of the alveoli

Structure	Level	Pattern	Image	Note
TS22: nasal septum mesenchyme	Present	Regionally restricted	5G/H	Abundant expression in mesenchyme within and adjacent to the perichondria of non-ossifying cartilage, including nasal septum.
TS22: olfactory epithelium	Absent		5G/H

Structure	Level	Pattern	Image	Note
TS24: heart	Present	Regionally restricted		Cardiac expression limited to mesenchymal condensations at the base of heart valves.
TS24: axial skeleton tail region	Present	Regionally restricted		Expression restricted to intervertebral disc anlagen; persists in caudal region up to 16.5 dpc.

Structure	Level	Pattern	Image	Note
TS24: limb	Ambiguous		3O

Structure	Level	Pattern	Image	Note
TS24: lung	Present	Regionally restricted	4K	Present in pulmonary mesothelia at all stages examined; epithelial expression is negligible.

Structure	Level	Pattern	Image	Note
TS24: otic cartilage	Present	Regionally restricted	5C	Abundant expression in mesenchyme within and adjacent to, the perichondria of non-ossifying cartilage including otic capsule (chondrifying peri-otic capsule).