Probe:	Tgfb2 probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: atrioventricular canal	Present	Regionally restricted	2A/B	Expression restricted to myocardium underlying cardiac jelly; hybridization in the region of the atrio-ventricular junction and in the myocardium of the outflow tract.
TS15: heart	Present	Regionally restricted	2A/B	Expression restricted to myocardium underlying cardiac jelly; hybridization in the region of the atrio-ventricular junction and in the myocardium of the outflow tract.
TS15: outflow tract	Present	Regionally restricted	2A/B	Expression restricted to myocardium underlying cardiac jelly; hybridization in the region of the atrio-ventricular junction and in the myocardium of the outflow tract.
TS15: common atrial chamber cardiac muscle	Absent		2A/B
TS15: primitive ventricle cardiac muscle	Absent		2A/B

Structure	Level	Pattern	Image	Note
TS15: neural tube	Absent		6A/B

Structure	Level	Pattern	Image	Note
TS17: heart	Present	Regionally restricted	2D/E	Expression in myocardium underlying cushion tissue, both in the atrio-ventricular region and underlying the bulbar cushion tissue of the outflow tract.
TS17: outflow tract	Present	Regionally restricted	2D/E	Expression in myocardium underlying cushion tissue, both in the atrio-ventricular region and underlying the bulbar cushion tissue of the outflow tract.
TS17: endocardial cushion	Present		2D/E	Some expression in cushion tissue per se.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Weak			Very low levels of hybridization.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		6J/K
TS17: fronto-nasal process	Present	Regionally restricted	6J/K	Expression in frontonasal mesenchyme.

Structure	Level	Pattern	Image	Note
TS18: neural tube	Present	Regionally restricted	6D/E	Transient appearance of low levels of transcripts in the ventral spinal cord between 10.5 and 11 dpc.
TS18: trunk sclerotome	Present	Regionally restricted	6D/E	Expression in chondrifying neural arch tissue.

Structure	Level	Pattern	Image	Note
TS20: limb	Strong	Regionally restricted	3K	High levels in precartilaginous blastemae.

Structure	Level	Pattern	Image	Note
TS20: lung	Present	Regionally restricted		Expression is limited to cuboidal epithelium of the growing terminal end buds and differentiating alveolar epithelium.
TS20: oral region	Present	Regionally restricted		Expression in the epithelium of the salivary gland prior to branching.

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Present	Regionally restricted	6G/H	No expression in the ventral spinal cord.

Structure	Level	Pattern	Image	Note
TS22: cardiac muscle tissue	Absent		2G/H	No myocardial expression.
TS22: heart valve	Present	Regionally restricted	2G/H	Transcripts detectable in the mesenchymal component of the maturing heart valves.

Structure	Level	Pattern	Image	Note
TS22: tail vertebral cartilage condensation	Absent		3B	No expression in mature cartilage. or in areas of intramembranous or endochondral ossification.

Structure	Level	Pattern	Image	Note
TS22: trachea mesenchyme	Absent		3F	No expression in mature cartilage. or in areas of intramembranous or endochondral ossification.

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Regionally restricted	4D	Expression limited to the cuboidal epithelium of the growing terminal end buds and differentiating alveolar epithelium; no expression in proximal columnar epithelium.

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Regionally restricted	4E	Expression limited to the cuboidal epithelium of the growing terminal end buds and differentiating alveolar epithelium; no expression in proximal columnar epithelium.

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Regionally restricted	4G	Expression limited to the cuboidal epithelium of the growing terminal end buds and differentiating alveolar epithelium; no expression in proximal columnar epithelium.

Structure	Level	Pattern	Image	Note
TS22: vibrissa	Present	Regionally restricted		Expression in invaginating whisker follicles.
TS22: salivary gland	Absent			No expression in the epithelium of the salivary glands when branching is in progress.
TS22: oral region	Present	Regionally restricted		Hybridization with invaginating dental epithelium.
TS22: inner ear	Strong	Regionally restricted		Sensory epithelial cells show intense hybridization.
TS22: neural retinal epithelium	Weak			Low levels within the outer neuroblastic layer of the retina.
TS22: lens anterior epithelium	Present			Expression in the anterior dividing epithelium of the lens.
TS22: eye	Strong	Regionally restricted		Intense expression in the region of the ciliary body and the ora serata.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	5E/F	Gradient of expression in epithelium lining the nasal septum and conchae; most intense on the distal (more differentiated) side of the pseudostratified epithelium.
TS22: vomeronasal organ epithelium	Present		5E/F

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	5I	Gradient of expression in epithelium lining the nasal septum and conchae; most intense on the distal (more differentiated) side of the pseudostratified epithelium.

Structure	Level	Pattern	Image	Note
TS24: heart valve	Present	Regionally restricted		Transcripts are still detectable in the mesenchymal component of the maturing heart valves.

Structure	Level	Pattern	Image	Note
TS24: limb	Present	Regionally restricted	3M/N	As development proceeds, expression becomes restricted to the proliferating chondroblast zone of the growth plate at the termini of long bones and digits.

Structure	Level	Pattern	Image	Note
TS24: lung	Present	Regionally restricted	4J	Expression limited to differentiating alveolar epithelium; not in the bronchi.

Structure	Level	Pattern	Image	Note
TS24: inner ear	Present	Regionally restricted	5A/B	Sensory epithelial cells show intense hybridization; no expression in the simple cuboidal epithelium lining the inner ear.
TS24: olfactory epithelium	Present	Regionally restricted	5A/B	Hybridization in the olfactory epithelium lining the nasal septum and conchae.

Structure	Level	Pattern	Image	Note
TS24: inner ear	Present	Regionally restricted	5D	Sensory epithelial cells show intense hybridization; no expression in the simple cuboidal epithelium lining the inner ear.
TS24: olfactory epithelium	Present	Regionally restricted	5D	Hybridization in the olfactory epithelium lining the nasal septum and conchae.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Weak		5J/K	Low levels within the outer neuroblastic layer of the retina.
TS24: lens anterior epithelium	Present		5J/K	Expression in the anterior dividing epithelium of the lens.
TS24: eye	Strong	Regionally restricted	5J/K	Intense expression in the region of the ciliary body and the ora serata.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Weak		5L	Low levels within the outer neuroblastic layer of the retina.
TS24: lens anterior epithelium	Present		5L	Expression in the anterior dividing epithelium of the lens.
TS24: eye	Strong	Regionally restricted	5L	Intense expression in the region of the ciliary body and the ora serata.