Gene Expression Data
Assay Details
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Assay
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Reference:
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J:11525
Monaghan AP, et al., The Msh-like homeobox genes define domains in the developing vertebrate eye. Development. 1991 Aug;112(4):1053-61
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2135584
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Gene symbol:
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Msx1
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Gene name:
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msh homeobox 1
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Probe:
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Probe 1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Authors did not state which of these two probes were used, this probe or a shorter probe (probe 2: MGI:1275945) or both. Probes were shortened to approximately a 100 bases by alkaline hydrolysis.
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Results
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Specimen
4A:
embryonic day 9.5
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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Swiss
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4C:
embryonic day 11.5
(more )
Specimen
4C:
(close )
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Genetic Background:
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Swiss
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4E:
embryonic day 12.5
(more )
Specimen
4E:
(close )
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Genetic Background:
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Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: retina |
Present |
Regionally restricted |
4E
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Expression near the tip of the optic cup in the inner layer, to the prospective region of the ciliary body. No expression at the tip or prospective iris tissue or in the prospective neural retina.
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Specimen
not shown:
embryonic day 13.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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either: C57BL/6 or Swiss
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: retina |
Present |
Regionally restricted |
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Transcripts were localized to a region that will give rise to the ciliary body.
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Specimen
5A, 5B:
embryonic day 15.5
(more )
Specimen
5A, 5B:
(close )
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Genetic Background:
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either: C57BL/6 or Swiss
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Regionally restricted |
5A
,
5B
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Expression is confined to the prospective ciliary body region as an assymmetrical band just below the tip of the optic cup.
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Specimen
5C:
embryonic day 16.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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either: C57BL/6 or Swiss
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: retina |
Present |
Regionally restricted |
5C
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Expression is showing an assymetrical pattern within the prospective ciliary body region just below the tip of the optic cup. Expression is at maximum strength at this stage.
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Specimen
5D, 5E:
embryonic day 19.5
(more )
Note: Fig. 5E is a higher magnification image of Fig. 5D.
Specimen
5D, 5E:
(close )
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Genetic Background:
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either: C57BL/6 or Swiss
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Age:
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embryonic day 19.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Fig. 5E is a higher magnification image of Fig. 5D.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: ciliary body |
Weak |
Regionally restricted |
5E
,
5D
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Expression in a diffuse band in the ciliary body.
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| TS26: iris |
Absent |
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5D
,
5E
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No expression in the developing retina or iris.
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| TS26: retina |
Absent |
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5D
,
5E
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No expression in the developing retina or iris.
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Analysis Tools