Probe:	Wnt1 cDNA4
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Absent

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	1A Wnt-1	Authors describe expression in the "neural tube" and "neural folds" in the region of the future brain "from E8.5 on."
TS12: future midbrain	Present	Regionally restricted	1A Wnt-1	Broad domain in the met-mesencephalic region.
TS12: future hindbrain	Present	Regionally restricted	1A Wnt-1	Two large triangular-shaped lateral patches of cells in "neural folds" on either side of the midline in the hindbrain.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	1B Wnt-1	Expression domains in 3 regions: (1) dorsal midline of neural tube, (2) ventral midline of mesencephalon and part of diencephalon, and (3) met-mesencephalic region.
TS15: diencephalon	Present	Regionally restricted	1B Wnt-1	Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. Expression on ventral midline of mes- and diencephalon.
TS15: future hindbrain	Present	Regionally restricted	1B Wnt-1	Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. Expression on ventral midline of mes- and diencephalon.
TS15: neural tube	Present	Regionally restricted	1B Wnt-1	Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. Expression on ventral midline of mes- and diencephalon.
TS15: future midbrain	Present	Regionally restricted	1B Wnt-1	Ring of cells, several cells wide, encircling the neural tube, rostral to the met-mesencephalic constriction. Expression on ventral midline of mes- and diencephalon.

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Present	Regionally restricted	2A Wnt-1	Expression domains in 3 regions: (1) dorsal midline of neural tube, (2) ventral midline of mesencephalon and part of diencephalon, and (3) met-mesencephalic region.
TS17: diencephalon	Present	Regionally restricted	2A Wnt-1	Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. On ventral midline, sharp limit at caudal end (Wnt1-positive ring); rostral extension reaches tuberculum posterium.
TS17: hindbrain	Present	Regionally restricted	2A Wnt-1	Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. On ventral midline, sharp limit at caudal end (Wnt1-positive ring); rostral extension reaches tuberculum posterium.
TS17: neural tube	Present	Regionally restricted	2A Wnt-1	Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. On ventral midline, sharp limit at caudal end (Wnt1-positive ring); rostral extension reaches tuberculum posterium.
TS17: midbrain	Present	Regionally restricted	2A Wnt-1	Ring of positive cells rostral to met-mesencephalic constriction; groove on ventricular surface of neuroepithelium marks caudal edge of ring. On ventral midline, sharp limit at caudal end; rostral extension reaches tuberculum posterius.

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Regionally restricted	2B Wnt-1	Expression domains in 3 regions: (1) dorsal midline of neural tube, (2) ventral midline of mesencephalon and part of diencephalon, and (3) met-mesencephalic region.
TS20: diencephalon	Present	Regionally restricted	2B Wnt-1	Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. On ventral midline, sharp limit at caudal end (Wnt1-positive ring); rostral extension depends on developmental stage.
TS20: hindbrain	Present	Regionally restricted	2B Wnt-1	Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. On ventral midline, sharp limit at caudal end (Wnt1-positive ring); rostral extension depends on developmental stage.
TS20: spinal cord	Present	Regionally restricted	2B Wnt-1	Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. On ventral midline, sharp limit at caudal end (Wnt1-positive ring); rostral extension depends on developmental stage.
TS20: cerebellum primordium	Present	Graded	2B Wnt-1	Expression on edge of cerebellar anlage decreases rostrally and medially. Authors state that the midline of the cerebellar edge remains negative at least until E11.5.
TS20: midbrain	Present	Regionally restricted	2B Wnt-1	Ring of positive cells rostral to met-mesencephalic constriction; groove on ventricular surface of neuroepithelium marks caudal edge of ring. On ventral midline, sharp limit at caudal end; rostral extension depends on developmental stage.

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Regionally restricted		Expression domains in 3 regions: (1) dorsal midline of neural tube, (2) ventral midline of mesencephalon and part of diencephalon, and (3) met-mesencephalic region.
TS20: diencephalon	Present	Regionally restricted		Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. On ventral midline, sharp limit at caudal end (Wnt1-positive ring); rostral extension depends on developmental stage.
TS20: hindbrain	Present	Regionally restricted		Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. On ventral midline, sharp limit at caudal end (Wnt1-positive ring); rostral extension depends on developmental stage.
TS20: spinal cord	Present	Regionally restricted		Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. On ventral midline, sharp limit at caudal end (Wnt1-positive ring); rostral extension depends on developmental stage.
TS20: midbrain	Present	Regionally restricted		Ring of positive cells rostral to met-mesencephalic constriction completely encircles the neural tube. On ventral midline, sharp limit at caudal end; rostral extension depends on developmental stage.

Structure	Level	Pattern	Image	Note
TS21: central nervous system	Present	Regionally restricted	2C Wnt-1	Expression domains in 3 regions: (1) dorsal midline of neural tube, (2) ventral midline of mesencephalon and part of diencephalon, and (3) met-mesencephalic region.
TS21: diencephalon	Present	Regionally restricted	2C Wnt-1	Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. On ventral midline, sharp limit at caudal end (Wnt1-positive ring); rostral extension depends on developmental stage.
TS21: hindbrain	Present	Regionally restricted	2C Wnt-1	Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. On ventral midline, sharp limit at caudal end (Wnt1-positive ring); rostral extension depends on developmental stage.
TS21: spinal cord	Present	Regionally restricted	2C Wnt-1	Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage. On ventral midline, sharp limit at caudal end (Wnt1-positive ring); rostral extension depends on developmental stage.
TS21: midbrain	Present	Regionally restricted	2C Wnt-1	Expression turned off in ventral and lateral regions of ring; persists in dorsal part of ring. On ventral midline, sharp limit at caudal end; rostral extension depends on developmental stage.

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Present	Regionally restricted		Expression domains in 3 regions: (1) dorsal midline of neural tube, (2) ventral midline of mesencephalon and part of diencephalon, and (3) met-mesencephalic region.
TS22: diencephalon	Present	Regionally restricted		Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage.
TS22: hindbrain	Present	Regionally restricted		Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage.
TS22: spinal cord	Present	Regionally restricted		Dorsal midline expression, from diencephalon to very caudal tip of spinal cord, with gap at cerebellar anlage.
TS22: midbrain	Present	Regionally restricted		On ventral midline, only in mesencephalon. Expression persists in dorsal part of ring.