Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:14355
Dono R, et al., The murine cripto gene: expression during mesoderm induction and early heart morphogenesis. Development. 1993 Aug;118(4):1157-68
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1934658
|
|
Gene symbol:
|
Cripto
|
|
Gene name:
|
cripto, EGF-CFC family member
|
|
Probe:
|
Cripto cDNA2
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
4A:
embryonic day 6.0-6.2
(more  )
Note: Prestreak.
Specimen
4A:
(close  )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 6.0-6.2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Prestreak.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS8: epiblast |
Present |
Regionally restricted |
4A/A'
|
Expression in a patch of epiblast cells in the region where embryonic ectoderm cells will invaginate to form the primitive streak.
|
Specimen
4B:
embryonic day 6.3-6.6
(more )
Note: Early streak.
Specimen
4B:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 6.3-6.6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Early streak.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo ectoderm |
Present |
Regionally restricted |
4B/B'
|
Expression in the most posterior part of the embryo, in the ectoderm at the site of early primitive streak formation, where cells are committed to form mesoderm.
|
| TS9: primitive streak |
Present |
Regionally restricted |
4B/B'
|
Expression in the most posterior part of the embryo, in the ectoderm at the site of early primitive streak formation, where cells are committed to form mesoderm.
|
Specimen
4C:
embryonic day 6.6-7.1
(more )
Note: Midstreak stage.
Specimen
4C:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 6.6-7.1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Midstreak stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo ectoderm |
Present |
Regionally restricted |
4C/C'
|
Expression in the ectoderm region proximal to the primitive streak and in all mesodermal cells.
|
| TS10: embryo mesoderm |
Present |
Regionally restricted |
4C/C'
|
Expression in the ectoderm region proximal to the primitive streak and in all mesodermal cells.
|
Specimen
4D:
embryonic day 6.6-7.1
(more )
Note: Midstreak stage.
Specimen
4D:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 6.6-7.1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Midstreak stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo ectoderm |
Present |
Regionally restricted |
4D/D'
|
Expression in the ectoderm region proximal to the primitive streak and in all mesodermal cells.
|
| TS10: embryo mesoderm |
Present |
Regionally restricted |
4D/D'
|
Expression in the ectoderm region proximal to the primitive streak and in all mesodermal cells.
|
Specimen
4E:
embryonic day 7.3-8.0
(more )
Note: Late streak.
Specimen
4E:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 7.3-8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Late streak.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
Regionally restricted |
4E/E'
|
Authors state that the pattern of expression is similar to that seen at the midstreak stage.
|
| TS11: embryo ectoderm |
Present |
Regionally restricted |
4E/E'
|
Expression in the ectodermal region proximal to the primitive streak and in all mesodermal cells.
|
| TS11: embryo mesoderm |
Present |
Regionally restricted |
4E/E'
|
Expression in the ectodermal region proximal to the primitive streak and in all mesodermal cells.
|
Specimen
4F:
embryonic day 7.3-8.0
(more )
Note: Late streak.
Specimen
4F:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 7.3-8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Late streak.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
Regionally restricted |
4F/F'
|
Authors state that the pattern of expression is similar to that seen at the midstreak stage.
|
| TS11: embryo ectoderm |
Present |
Regionally restricted |
4F/F'
|
Expression in the ectodermal region proximal to the primitive streak and in all mesodermal cells.
|
| TS11: embryo endoderm |
Present |
Regionally restricted |
4F/F'
|
Expression in the ectodermal region proximal to the primitive streak and in all mesodermal cells.
|
| TS11: notochordal process |
Present |
|
4F/F'
|
Expression detected in the head process region.
|
Specimen
5A:
embryonic day 6.3-6.6
(more )
Note: Early streak.
Specimen
5A:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 6.3-6.6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Early streak.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo ectoderm |
Present |
Regionally restricted |
5A/A'
|
Expression in the most posterior part of the embryo, in the ectoderm at the site of early primitive streak formation, where cells are committed to form mesoderm.
|
| TS9: primitive streak |
Present |
Regionally restricted |
5A/A'
|
Expression in the most posterior part of the embryo, in the ectoderm at the site of early primitive streak formation, where cells are committed to form mesoderm.
|
Specimen
5B:
embryonic day 6.6-7.1
(more )
Note: Midstreak stage.
Specimen
5B:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 6.6-7.1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Midstreak stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo ectoderm |
Present |
Regionally restricted |
5B/B'
|
Expression in the ectoderm region proximal to the primitive streak and in all mesodermal cells.
|
| TS10: embryo mesoderm |
Present |
Regionally restricted |
5B/B'
|
Expression in the ectoderm region proximal to the primitive streak and in all mesodermal cells.
|
| TS10: primitive streak |
Present |
Regionally restricted |
5B/B'
|
Strong expression in cells migrating through the primitive streak.
|
Specimen
5C:
embryonic day 6.6-7.1
(more )
Note: Midstreak stage.
Specimen
5C:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 6.6-7.1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Midstreak stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS10: embryo ectoderm |
Present |
Regionally restricted |
5C/C'
|
Expression in the ectoderm region proximal to the primitive streak and in all mesodermal cells.
|
| TS10: embryo mesoderm |
Present |
Regionally restricted |
5C/C'
|
Expression in the ectoderm region proximal to the primitive streak and in all mesodermal cells.
|
| TS10: primitive streak |
Strong |
Regionally restricted |
5C/C'
|
Strong expression in cells migrating through the primitive streak.
|
Specimen
5D:
embryonic day 7.3-8.0
(more )
Note: Late streak.
Specimen
5D:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 7.3-8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Late streak.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
Regionally restricted |
5D/D'
|
Authors state that the pattern of expression is similar to that seen at the midstreak stage.
|
| TS11: primitive streak |
Strong |
Regionally restricted |
5D/D'
|
Strong expression in cells migrating through the primitive streak.
|
Specimen
6D:
embryonic day 9.5
(more )
Specimen
6D:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: outflow tract |
Present |
Regionally restricted |
6D/D'
|
Expression only in the truncus arteriosus.
|
Specimen
6E:
embryonic day 10.5
(more )
Specimen
6E:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: outflow tract |
Present |
Regionally restricted |
6E/E'
|
Expression only in the truncus arteriosus.
|
Specimen
7A:
embryonic day 7.3-8.0
(more )
Note: Late streak.
Specimen
7A:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 7.3-8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Late streak.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
Regionally restricted |
7A/A'
|
Authors state that the pattern of expression is similar to that seen at the midstreak stage.
|
| TS11: notochordal process |
Present |
|
7A/A'
|
Expression detected in the head process region.
|
Specimen
7B:
embryonic day 8.5
(more )
Specimen
7B:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: outflow tract |
Present |
Regionally restricted |
7B/B'
|
Expression restricted to the truncus arteriosus of the heart. All other mesodermal derivatives are negative.
|
Specimen
7C:
embryonic day 9.1
(more )
Specimen
7C:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 9.1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: outflow tract |
Present |
Regionally restricted |
7C/C'
|
Expression localized in the cells of the developing truncus arteriosus.
|
|
Analysis Tools