Gene Expression Data
Assay Details
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Assay
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Reference:
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J:31212
Shimamura K, et al., Longitudinal organization of the anterior neural plate and neural tube. Development. 1995 Dec;121(12):3923-33
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1934645
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Gene symbol:
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Otx1
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Gene name:
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orthodenticle homeobox 1
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Probe:
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Otx1 probe5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6 G:
embryonic day 8.0
(more  )
Note: 7 somites.
Specimen
6 G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7 somites.
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Specimen
not shown:
embryonic day 8.0
(more )
Note: 2-3 somites.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-3 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future midbrain |
Present |
Regionally restricted |
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At the 2-3-somite stage expression is in a transverse band in the region of the presumptive forebrain and midbrain.
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| TS12: future prosencephalon |
Present |
Regionally restricted |
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At the 2-3-somite stage expression is in a transverse band in the region of the presumptive forebrain and midbrain.
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Specimen
6 H:
embryonic day 8.5
(more )
Note: 10 somites.
Specimen
6 H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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10 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future hindbrain |
Present |
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6H
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The rostral limit of expression has a sharp boundary that surrounds caudolaterally the incipient optic cup and expression extends down to the presumptive midbrain-hindbrain boundary.
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| TS13: future midbrain |
Present |
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6H
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The rostral limit of expression has a sharp boundary that surrounds caudolaterally the incipient optic cup and expression extends down to the presumptive midbrain-hindbrain boundary.
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| TS13: future prosencephalon |
Present |
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6H
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The rostral limit of expression has a sharp boundary that surrounds caudolaterally the incipient optic cup and expression extends down to the presumptive midbrain-hindbrain boundary.
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Specimen
6 I:
embryonic day 10.5
(more )
Specimen
6 I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forebrain |
Present |
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6I
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Sharp transverse boundaries of expression within the secondary prosencephalon and at the isthmus.
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| TS17: midbrain |
Present |
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6I
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Sharp transverse boundaries of expression within the secondary prosencephalon and at the isthmus.
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Analysis Tools